Interactions between the tumor and the blood systemic response of breast cancer patients.

Although systemic immunity is critical to the process of tumor rejection, cancer research has largely focused on immune cells in the tumor microenvironment. To understand molecular changes in the patient systemic response (SR) to the presence of BC, we profiled RNA in blood and matched tumor from 173 patients. We designed a system (MIxT, Matched Interactions Across Tissues) to systematically explore and link molecular processes expressed in each tissue.

Peripheral blood cells inform on the presence of breast cancer: a population-based case-control study.

Tumor-host interactions extend beyond the local microenvironment and cancer development largely depends on the ability of malignant cells to hijack and exploit the normal physiological processes of the host. Here, we established that many genes within peripheral blood cells show differential expression when an untreated breast cancer (BC) is present, and harnessed this fact to construct a 50-gene signature that distinguish BC patients from population-based controls. Our results were derived from a series of large datasets within our unique population-based Norwegian Women and Cancer cohort that allowed us to investigate the influence of medications and tumor characteristics on our blood-based test, and were further tested in two external datasets.

Do static or time-varying magnetic fields in magnetic resonance imaging (3.0 T) alter protein-gene expression?-A study on human embryonic lung fibroblasts.

Purpose: To evaluate the influence of magnetic resonance imaging (MRI) on gene expression in embryonic human lung fibroblasts (Hel 299).

Materials And Methods: The cells were exposed to the static magnetic field and to a turbo spin-echo sequence of an MR scanner at 3.0 Tesla.

Detection of DNA double-strand breaks using gammaH2AX after MRI exposure at 3 Tesla: an in vitro study.

Purpose: To evaluate the effects of the static magnetic field and typical imaging sequences of a high-field magnetic resonance scanner (3 Tesla) on the induction of double-strand breaks (DSBs) in two different human cell lines.

Materials And Methods: Human promyelocytic leukemia cells (HL-60) and human acute myeloid leukemia cells (KG-1a) were exposed to the static magnetic field alone and to turbo spin-echo (TSE) and gradient-echo (GE) sequences. Flow cytometry was used to quantify gammaH2AX (serine 139 phosphorylated form of histone H2AX) expression of antibody-stained cells as a marker for deoxyribonucleic acid (DNA) DSBs one hour and 24 hours after magnetic field exposure.

In vitro evaluation of magnetic resonance imaging at 3.0 tesla on clonogenic ability, proliferation, and cell cycle in human embryonic lung fibroblasts.

Objectives: We investigated the influence of magnetic resonance (MR) at 3.0 T on clonogenic ability, proliferation, and cell cycle in an embryonic human cell line.

Materials And Methods: Cells (human lung fibroblasts Hel 299) were exposed to the static magnetic field (3.

3D imaging of the whole spine at 3T compared to 1.5T: initial experiences.

Purpose: To describe our first experiences with a recently introduced 3T system for T2-weighted isotropic 3D whole-spine imaging.

Material And Methods: Magnetic resonance imaging of the whole spine was performed by implementing an isotropic 3D fast spin-echo sequence with variable flip-angle refocusing pulses at 3T and 1.5T.

MRI-compatible incubation chamber for cell culture experiments.

Purpose: To develop an incubation chamber that is compatible with MRI, while avoiding sources of error due to the experimental setup.

Materials And Methods: Two identical and gas-tight chambers were constructed of Plexiglas. The temperature and the CO(2) concentration were adjustable.

[Liposome-mediated transfection of thrombomodulin in vascular smooth muscle cells--a gene therapy concept to inhibit recurrent stenosis].

Purpose: Thrombomodulin (TM), an integral endothelial receptor, is known for its anticoagulant functions. Moreover, there is evidence of growth-modulating effects of this cell surface -protein. The aim of our study was to establish by in vitro transfection a stable cell line of vascular smooth muscle cells with overexpression of TM for further investigations concerning the influence of TM on cellular proliferation and its potential role during the formation of restenosis.

Subcapsular liver hematoma in HELLP syndrome: Evaluation of diagnostic and therapeutic options--a unicenter study.

Objective: Subcapsular liver hematoma formation has been reported in less than 2% of pregnancies complicated by HELLP (hemolysis, elevated liver enzymes, low platelets) syndrome. The purpose of this study was to identify the main diagnostic and therapeutic options for management of these patients.

Study Design: In this 10-year retrospective review, we performed a computer-directed search of all cases of confirmed HELLP syndrome with hepatic hematoma treated in the surgical department of our tertiary care referral medical center.

Impact of glafenine hydrochloride on human endothelial cells and human vascular smooth muscle cells: a substance reducing proliferation, migration and extracellular matrix synthesis.

The aim of this study was to examine the effects of glafenine hydrochloride (a nonsteroidal anti-inflammatory drug) on proliferation, clonogenic activity, cell-cycle, migration, and the extracellular matrix protein tenascin of human aortic smooth muscle cells (haSMCs) and human endothelial cells (ECs) in vitro.HaSMCs and ECs were seeded in tissue culture flasks. The cells were treated for 4 days with glafenine hydrochloride (10 microM, 50 microM, 100 microM).

Antiproliferative effects of the antiallergic agent azelastine on human aortic smooth-muscle cells: an in vitro study.

Rationale And Objectives: The aim of the study was to examine the effects of azelastine on proliferation, clonogenic activity, cell-cycle, and migration of human aortic smooth-muscle cells (haSMCs) in vitro.

Methods: HaSMCs were treated for 4 days with azelastine (1 micromol/L, 25 micromol/L, 50 micromol/L). Half of the treated groups were incubated again with azelastine, the other half received azelastine-free medium every 4 days until day 20.

Meclofenamic acid for inhibition of human vascular smooth muscle cell proliferation and migration: an in vitro study.

Purpose: The aim of the study was to examine the effects of meclofenamic acid on proliferation, clonogenic activity, migratory ability, cell cycle distribution and p44/42 MAPK (mitogen activated protein kinase) expression in serum-stimulated human aortic smooth muscle cells (haSMCs).

Methods: haSMCs were treated with meclofenamic acid in three different concentrations (10 mM, 100 mM, 200 mM) for 4 days. Then meclofenamic acid-free culture medium was supplemented until day 20.

Flufenamic acid: growth modulating effects on human aortic smooth muscle cells in vitro.

Purpose: The aim of the study was to examine the effects of flufenamic acid on proliferation, clonogenic activity, migratory ability, cell-cycle distribution, and p44/42-mitogen-activated protein kinase (MAPK) expression on serum-stimulated human aortic smooth muscle cells (haSMCs) in vitro.

Materials And Methods: HaSMCs were treated with flufenamic acid in three different doses (40 micromol/L, 200 micromol/L, 400 micromol/L) for 4 days, and then flufenamic-acid-free culture medium was supplemented every 4 days until day 20 after initial treatment. The growth kinetics were assessed.

Biological effects of ionizing radiation on human blood compounds ex vivo.

Purpose: Blood compounds are irradiated ex vivo to prevent transfusion-associated graft-versus-host-disease. Recently, ex vivo irradiation of re-transfused wound blood has been proposed to prevent metastatic spread in patients with malignant tumors, an issue requiring different dose concepts. To determine effects on blood cells we examined the impact of various doses of ionizing radiation.

In vitro evaluation of teratogenic effects by time-varying MR gradient fields on fetal human fibroblasts.

The purpose of this study was to evaluate the influence on fetal cell growth in vitro of rapidly changing magnetic gradient fields such as those produced by the gradient coils of a typical magnetic resonance (MR) imager. The static magnetic field and the radiofrequency pulses were disabled during all measurements. Human fetal fibroblasts were placed within a specially designed MR-compatible incubation system inside the magnet.

Human fetal lung fibroblasts: in vitro study of repetitive magnetic field exposure at 0.2, 1.0, and 1.5 T.

Purpose: To evaluate whether repetitive exposure to magnetic fields of 0.2, 1.0, and 1.