Cyclophosphamide Treatment Mimics Sub-Lethal Infections With in Immunocompromised Individuals.

Microsporidia, including , are emerging pathogens which cause opportunistic infections in immunocompromised patients, such as those with AIDS, cancer, the elderly and people on immunosuppressive drugs. Intestinal mucosa (IM) is crucial for developing an efficient adaptive immune response against pathogenic micro-organisms, thereby preventing their colonization and subsequent infection. As immunosuppressive drugs affect the intestinal immune response is little known.

Stress and overweight/obesity among nursing students.

Objective: analyze associations between demographic, academic, health, stress, overweight and obesity characteristics among nursing students.

Method: this is a cross-sectional study with 95 students from a private university in Rio Grande do Sul, Brazil. A demographic, academic and health characterization questionnaire and the Assessment of Stress in Nursing Students (ASNS) scale were applied.

Medicinal Plants from Brazilian Cerrado: Antioxidant and Anticancer Potential and Protection against Chemotherapy Toxicity.

The use of natural antioxidants in cancer therapy has increased: first, due to the potential of natural antioxidants to kill tumour cells and second, because of their capacity to protect healthy cells from the damage caused by chemotherapy. This review article discusses the antioxidant properties of extracts obtained from medicinal plants from the Brazilian Cerrado and the cell death profile induced by each of these extracts in malignant cells. Next, we describe the capacity of other medicinal plants from the Cerrado to protect against chemotherapy-induced cell toxicity.

CTX-M- and pAmpC-Encoding Genes Are Associated with Similar Mobile Genetic Elements in Isolated from Different Brands of Brazilian Chicken Meat.

In this study we characterized the genetic environment of and genes carried by 46 isolates obtained from 20 chicken carcasses produced by five different brands in Brazil, including exporters and antibiotic-free-certified producers, purchased between 2010 and 2014. Similar plasmids characterized according to size and incompatibility group (Inc) were identified in belonging to different MLST-ST collected, regardless of carcass brand or production system. Hybridization assays with transconjugant strains revealed that gene ( = 19) was located on 85 kb plasmids of IncB/O, IncI1, IncFIB, or nontypeable groups.

Usefulness of raw bioelectrical impedance parameters in tracking fluid shifts in judo athletes.

Bioelectrical impedance (BI) has been widely used but clarification about the behaviour of raw BI measurements under specific athletic conditions is required. Thus, we determined the usefulness of raw BI measures in tracking body fluids changes during the preparation period prior to competition in elite Judo athletes. At baseline (weight stability), 27 male athletes were evaluated (23.

Chemobrain in rats: Behavioral, morphological, oxidative and inflammatory effects of doxorubicin administration.

Doxorubicin (DOX) is known to cause cognitive impairments in patients submitted to long-term chemotherapy (deficits also known as chemobrain). The present study investigated whether DOX administration could affect behavior and brain morphology, as well as oxidative and inflammatory status in rats. Male Wistar rats were injected with DOX (2.

Hormonal and Psychogenic Risk Factors for Erectile Dysfunction in Men with HTLV-1.

Introduction: Erectile dysfunction (ED) is associated with neurological damage due to human T-lymphotropic virus 1 (HTLV-1) infection, but hormonal and psychogenic factors also cause ED.

Aim: To evaluate the association of psychogenic and hormonal factors with ED in men infected with HTLV-1.

Methods: In this cross-sectional study, we compared total testosterone, follicle stimulating hormone, luteinizing hormone, prolactin, anxiety symptoms, depressive symptoms, and neurologic manifestations in HTLV-1-infected men with or without ED.

GATA3-Controlled Nucleosome Eviction Drives Enhancer Activity in T-cell Development and Leukemia.

Long-range enhancers govern the temporal and spatial control of gene expression; however, the mechanisms that regulate enhancer activity during normal and malignant development remain poorly understood. Here, we demonstrate a role for aberrant chromatin accessibility in the regulation of expression in T-cell lymphoblastic leukemia (T-ALL). Central to this process, the NOTCH1- enhancer (N-Me), a long-range T cell-specific enhancer, shows dynamic changes in chromatin accessibility during T-cell specification and maturation and an aberrant high degree of chromatin accessibility in mouse and human T-ALL cells.

Is there an indication of the origin of nutrient supply in different morphological structures of macrofauna at two different Brazilian southeastern sandy beaches? Comparison by C and N stable isotopes.

The goals of this study were to analyze if there is a difference in the stable isotopic ratio (δC and δN) of macrobenthic species sampled at two sandy beaches (one close to a river mouth and the other far from any freshwater input) and to identify differences in the stable isotopic ratio (δC and δN) in different body parts of three representative species of two Brazilian sandy beach macrofaunas: the polychaete Hemipodia californiensis, the mollusk bivalve Donax hanleyanus, and the crustacean decapod Emerita brasiliensis. No significant differences were detected in the δC stable isotopic ratio between the two sites analyzed; however, in the δN stable isotopic ratio, a significant difference was observed. Regarding the intraspecific response of stable isotopic ratio, D.

Values of plasma total proteins of free-living marmosets (Callithrix jacchus) in an urban forest.

Background: Callithrix jacchus is a small primate widely used in experimentation, but data on plasma total protein (PTP) values of free-living animals inhabiting its own endemism region are non-existent.

Methods: Marmosets belonging to two free-living groups were captured for marking and obtaining biological material. The collected blood was centrifuged to obtain the plasma and thus to determine the concentration of PTP.

Ultrasensitive System for Electrophysiology of Cancer Cell Populations: A Review.

Bioelectricity is the electrical activity produced by living organisms. Understanding the role of bioelectricity in a disease context is important as it contributes to both disease diagnosis and therapeutic intervention. Electrophysiology tools work well for neuronal cultures; however, they are limited in their ability to detect the electrical activity of non-neuronal cells, wherein the majority of cancers arise.

ARTHROSCOPIC TREATMENT FOR LATERAL EPICONDYLITIS: OUTCOMES IN 104 CASES OF A SINGLE INSTITUTION.

Objective: To evaluate the outcomes of patients that underwent arthroscopic surgery for lateral epicondylitis (LE), after failed conservative treatment.

Methods: One hundred four patients with LE treated with arthroscopic debridement of the extensor carpi radialis brevis (ECRB) tendon were enrolled in this retrospective study. They were evaluated using Disabilities of the Arm, Shoulder and Hand (DASH) score, Visual Analogue Scale (VAS) and Short Form Health Survey (SF-36) scale.

Safety culture of multidisciplinary teams from neonatal intensive care units of public hospitals.

Objective: analyze the safety culture of multidisciplinary teams from three neonatal intensive care units of public hospitals in Minas Gerais, Brazil.

Method: a cross-sectional survey conducted with 514 health professionals, using the Hospital Survey on Patient Safety Culture; data were subjected to a descriptive statistical analysis in software R-3.3.

Maisonneuve Variant Lesion with Proximal Tibiofibular Dislocation.

Maisonneuve lesion is a rare entity, accounting for 7% of all ankle fractures. One of its variants includes a proximal tibiofibular dislocation, which is an even more unusual injury. This article reports the case of a 34-year-old male patient admitted to the emergency room with left lower limb edema after a sports trauma.

Determination of antipsychotic drugs in oral fluid using dried saliva spots by gas chromatography-tandem mass spectrometry.

The present work describes the optimization and validation of an analytical method for the determination of six antipsychotic drugs (chlorpromazine, levomepromazine, cyamemazine, clozapine, haloperidol, and quetiapine) in oral fluid samples after solvent extraction from dried saliva spots, by gas chromatography coupled to tandem mass spectrometry. The method was fully validated, and the included parameters were selectivity, linearity, limits of quantification, precision and accuracy, stability, and recovery. The method was linear for all compounds from 10 to 400 ng/mL, except for haloperidol (5-100 ng/mL), presenting coefficients of determination higher than 0.

Updates on medical management of hyperkalemia.

Purpose Of Review: Hyperkalemia is a potentially fatal electrolyte disorder, more commonly present when the potassium excretion capacity is imparied. Hyperkalemia can lead to adverse outcomes, especially due to severe cardiac arrhythmias. It can also impair the cardiovascular effects of renin-angiotensin-aldosterone system inhibitors (RAASis) and potassium rich diets, as hyperkalemia frequently leads to their discontinuation.

Dermal-Fat Graft for Facial Contouring in Patients With Craniofacial Microsomia.

Craniofacial microsomia (CFM) is a variable craniofacial malformation, related to the development of the structures originated from the first and second brachial arches, affecting skeletal tissue, soft tissue, and neuromuscular components. In the situation of subcutaneous tissue and chewing muscles hypoplasia, free tissue transfer is a treatment option. Dermal-fat graft allows easy modeling during surgery, volumetric gain and improvement of asymmetry.

Serum cytokine levels as predictive biomarkers of benefit from ipilimumab in small cell lung cancer.

. Immunotherapy has shown efficacy in small cell lung cancer (SCLC), but only a subset of patients benefits. Surrogate biomarkers are urgently needed.

Determination of amphetamine-type stimulants in urine samples using microextraction by packed sorbent and gas chromatography-mass spectrometry.

The aim of this work was the development, optimization and full validation of a method applying microextraction by packed sorbent (MEPS) coupled to gas chromatography-mass spectrometry (GC-MS) to determine amphetamine (AMP), methamphetamine (MAMP), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxyethylmethamphetamine (MDMA), 3,4-methylenedioxy-N-methyl-α-ethylfenilethylamine (MBDB), and 3,4-methylenedioxy-N-ethylamphetamine (MDE) in urine samples. Using 200 μL of sample, the MEPS procedure was optimized concerning type of sorbent, sample dilution, number of strokes, activation of the ion exchange mechanism and composition of both washing and elution solvents. The method was fully validated according to the Food and Drug Administration and the Scientific Working Group of Forensic Toxicology guidelines for the validation of bioanalytical methods.

Communication for patient safety in pediatric hospitalizations.

Objective: To analyze the perception of health professionals and companions/family about the development of communication for patient safety in pediatric hospitalizations.

Method: It is an exploratory-descriptive study with a qualitative approach, performed in pediatric clinical-surgical hospitalization units of three hospitals in Porto Alegre, RS, Brazil. 44 health professionals and 94 companions of hospitalized children participated in the study, to a total of 138 participants.

NEOTROPICAL XENARTHRANS: a data set of occurrence of xenarthran species in the Neotropics.

Xenarthrans-anteaters, sloths, and armadillos-have essential functions for ecosystem maintenance, such as insect control and nutrient cycling, playing key roles as ecosystem engineers. Because of habitat loss and fragmentation, hunting pressure, and conflicts with domestic dogs, these species have been threatened locally, regionally, or even across their full distribution ranges. The Neotropics harbor 21 species of armadillos, 10 anteaters, and 6 sloths.

Next-generation Sequencing for ALK and ROS1 Rearrangement Detection in Patients With Non-small-cell Lung Cancer: Implications of FISH-positive Patterns.

Background: Detection of ALK and ROS1 gene rearrangements in non-small-cell lung cancer is required for directing patient care. Although fluorescence in situ hybridization (FISH) and immunohistochemistry have been established as gold standard methods, next-generation sequencing (NGS) platforms are called to be at least equally successful. Comparison of these methods for translation into daily use is currently under investigation.

Almost a hundred years later, the advertisement call of Aparasphenodon brunoi Miranda-Ribeiro 1920 (Anura: Hylidae) from the Atlantic Forest.

The Neotropical genus Aparasphenodon Miranda-Ribeiro is composed of five recognized species commonly referred as "casque-headed frogs" (Frost 2018). Aparasphenodon brunoi Miranda-Ribeiro occurs in Atlantic Forest lowlands from the states of São Paulo to Bahia, Brazil (Feio et al. 1998; Mollo Neto Teixeira Jr.

Arch of cricoid cartilage anatomical variation: morphological and radiological aspects.

The cricoid cartilage serves as an anatomical reference for several surgical procedures for access to the airway. Additionally, it serves as an attachment point for muscles that move the vocal folds. We present a case where the cricoid cartilage arch is divided into distinct superior and inferior arches, with a fibrous membrane between them.