LC-ESI-HRMS - lipidomics of phospholipids : Characterization of extraction, chromatography and detection parameters.

Lipids are a diverse class of molecules involved in many biological functions including cell signaling or cell membrane assembly. Owing to this relevance, LC-MS/MS-based lipidomics emerged as a major field in modern analytical chemistry. Here, we thoroughly characterized the influence of MS and LC settings - of a Q Exactive HF operated in Full MS/data-dependent MS TOP N acquisition mode - in order to optimize the semi-quantification of polar lipids.