Femoral tunnel enlargement after medial patellofemoral ligament reconstruction is common and can be monitored using plain radiographs.

Introduction: medial patellofemoral ligament reconstruction (MPFLr) is a common surgical procedure for treating patellar instability. Grafts can be fixed to the femur using a bone-tunnel technique with an interference screw. However, this may lead to femoral tunnel enlargement (FTE) post-operatively.

An extracellular vesicle delivery platform based on the PTTG1IP protein.

Extracellular vesicles (EVs) are promising therapeutic delivery vehicles, although their potential is limited by a lack of efficient engineering strategies to enhance loading and functional cargo delivery. Using an in-house bioinformatics analysis, we identified N-glycosylation as a putative EV-sorting feature. PTTG1IP (a small, N-glycosylated, single-spanning transmembrane protein) was found to be a suitable scaffold for EV loading of therapeutic cargoes, with loading dependent on its N-glycosylation at two arginine residues.

Mechanical properties of snake skin vary longitudinally, following large prey ingestion and among species.

The ability for snakes to ingest large prey (macrostomy) is a widespread, derived trait that involves distending the skin during ingestion and metabolic upregulation during digestion. The material behavior of the skin must accommodate significant stretch associated with a large prey bolus, but data remain sparse for how the material properties of snake skin vary: longitudinally within an individual, after ingesting large prey and among species. To test whether these three factors affected the mechanical properties of snake skin, we quantified uniaxial stresses and strains in circumferential loops of skin from the neck, mid-body and tail of fasted and recently fed Boa constrictor.

Sustainable antimicrobial resistance surveillance: time for a global funding mechanism.

Antimicrobial resistance (AMR) is predicted to outstrip malaria, HIV, and tuberculosis combined as the leading infectious cause of death by 2050. Strengthening the knowledge and evidence base for AMR with surveillance and research is one of the five main objectives of the WHO Global Action Plan on AMR. While recent efforts to strengthen diagnosis and surveillance have been encouraging, these are unlikely to be sustainable without continued funding support in most low-resource settings.

Recommendations for the optimal introduction of novel antibiotics to treat uncomplicated gonorrhoea in the face of increasing antimicrobial resistance: a case study with zoliflodacin.

New, first-in-class oral antibiotics like zoliflodacin, developed in a public-private partnership, require an optimal introduction strategy while ensuring antibiotic stewardship. Zoliflodacin, given as a single dose for uncomplicated urogenital gonorrhoea, recently demonstrated non-inferiority to ceftriaxone plus azithromycin and safety in a phase 3 randomised controlled trial. Following regulatory approval, zoliflodacin could improve sexually transmitted infection (STI) management and help address the threat of untreatable gonorrhoea, as levels of resistance to current first-line treatments increase.

Cost-effectiveness of long-acting progestogens versus the combined oral contraceptives pill for preventing recurrence of endometriosis-related pain following surgery: an economic evaluation alongside the PRE-EMPT trial.

Objectives: To evaluate the cost-effectiveness of long-acting progestogens (LAP), including levonorgestrel-releasing intrauterine system (LNG-IUS) and depot-medroxyprogesterone acetate (DMPA), compared with the combined oral contraceptives pill (COCP) in preventing recurrence of endometriosis-related pain postsurgery.

Design: Within-trial economic evaluation alongside a multicentre, pragmatic, parallel-group, open-label, randomised controlled trial (Preventing Recurrence of Endometriosis by means of Long-Acting Progestogen Therapy trial).

Setting: Thirty-four UK hospitals recruiting participants from November 2015 to March 2019.

Delta wave MRI - fMRI imaging of electrophysiological activity.

We propose a novel application of MR encephalography (MREG) to detect the frequency spectrum of endogenous slow oscillatory brain activity (delta, <4 Hz)[1]. MREG offers faster image acquisition than conventional fMRI and superior spatial localization than EEG/MEG. MREG was acquired at 0.

Immediate postinjury extracorporeal carbon dioxide removal reduces ventilator requirements and mitigates acute respiratory distress syndrome in swine.

Background: Awareness of ventilator-induced lung injury contributed to increased use of extracorporeal interventions, but not immediately after injury, before acute respiratory distress syndrome (ARDS) ensues. Our objective was to evaluate the role of venovenous extracorporeal carbon dioxide removal (ECCO2R) in management of mechanically ventilated swine with smoke inhalation injury and 40% body surface area burns.

Methods: Yorkshire swine (n = 29, 43.

AmpC β-lactamases detected in Southeast Asian and .

Objectives: AmpC β-lactamases are neglected compared with ESBL as a cause of third-generation cephalosporin (3GC) resistance in Enterobacterales in low- and middle-income countries and the burden is unknown. The aim of this study was to investigate the presence of AmpC β-lactamase-producing and in clinical specimens from three clinical research laboratories in Southeast Asia.

Methods: Stored clinical isolates of and resistant to ceftriaxone or ceftazidime or cefpodoxime and ESBL confirmation test negative were screened using MASTDISCS AmpC, ESBL and Carbapenemase Detection Set-D72C.

Pinpointing the timing of meiosis: a critical factor in evaluating the impact of abiotic stresses on the fertility of cereal crops.

The development of male gametes, vital to sexual reproduction in crops, requires meiosis followed by successive mitotic cell divisions of haploid cells. The formation of viable pollen is especially vulnerable to abiotic stress, with consequences both for yield and for grain quality. An understanding of key molecular responses when specific stages during pollen development are subjected to stress (e.

Towards Biomarkers for Autism Spectrum Disorder: Contributions of Magnetoencephalography (MEG).

There is no simple blood test for autism. Consequently, much attention has been paid to identifying noninvasive biomarkers using imaging (e.g.

ERK-dependent protein phosphorylation in KRAS-mutant cancer: a mix of the expected and surprising.

Recently developed KRAS inhibitors have delivered clinical benefits but their antitumor efficacy remains limited. A recent study by Klomp et al. reports an unprecedentedly comprehensive profiling of protein phosphorylation dependent on the KRAS pathway and generates new insights and directions to improve the efficacy of KRAS-targeted therapies.

GABA and glutamate measurements in temporal cortex of autistic children.

Autism spectrum disorder (ASD) is a neurodevelopmental disorder and presents with challenges in social communication. A hypothesized underlying contributing mechanism is the imbalance in excitation and inhibition (E/I), partly influenced by the levels of excitatory neurotransmitter glutamate (Glu) and inhibitory neurotransmitter γ-aminobutyric acid (GABA) in the brain. Although many have reported the levels of GABA and Glu in the brain, only a few reports address the temporal cortex and then only with a small sample of autistic children, and often only in one hemisphere.

Treatment of adult spine deformity: A retrospective comparison of bone morphogenic protein and bone marrow aspirate with bone allograft.

Unlabelled: BACKGROUND  : The use of bone morphogenic protein (BMP-2) in adult spine deformity (ASD) surgery remains controversial more than two decades following its approval for clinical application in spine surgery. This study was performed to assess outcomes in patients undergoing ASD surgery with BMP application compared with a combination of bone marrow aspirate, cancellous bone chips and i-Factor.

Methods: This was a retrospective cohort study.

Cross-Validation of Equations for Estimating 1 Repetition Maximum From Repetitions to Failure for the Bench Press and Leg Extension.

Roberts, TD, Smith, RW, Arnett, JE, Ortega, DG, Schmidt, RJ, and Housh, TJ. Cross-validation of equations for estimating 1 repetition maximum from repetitions to failure for the bench press and leg extension. J Strength Cond Res XX(X): 000-000, 2024-Eighteen previously published equations (EQs) that estimate 1 repetition maximum (1RM) from repetitions to failure (RTF) were cross-validated for the bench press (BP) and leg extension (LE) movements.

Accommodative and Vergence Responses to a Moving Stimulus in Concussion.

Purpose: Concussed adolescents often report visual symptoms, especially for moving targets, but the mechanisms resulting in oculomotor deficits remain unclear. We objectively measured accommodative and vergence responses to a moving target in concussed adolescents and controls.

Methods: Thirty-two symptomatic concussed participants (mean age, 14.

Systematic analysis of tRNA transcription unit deletions in E. coli reveals insights into tRNA gene essentiality and cellular adaptation.

Transfer ribonucleic acids (tRNAs) are essential for protein synthesis, decoding mRNA sequences into amino acids. In E. coli K-12 MG1655, 86 tRNA genes are organized in 43 transcription units (TUs) and the essentiality of individual tRNA TUs in bacterial physiology remains unclear.

Evolving Escherichia coli to use a tRNA with a non-canonical fold as an adaptor of the genetic code.

All known bacterial tRNAs adopt the canonical cloverleaf 2D and L-shaped 3D structures. We aimed to explore whether alternative tRNA structures could be introduced in bacterial translation. To this end, we crafted a vitamin-based genetic system to evolve Escherichia coli toward activity of structurally non-canonical tRNAs.