Characteristics of a Spray-Dried Porcine Blood Meal for Mosquitoes.

Research into mosquito-borne illnesses faces hurdles because feeding fresh animal blood to rear female mosquitoes presents logistical, economic, and safety challenges. In this study, a shelf-stable additive (spray-dried porcine blood; SDPB) hypothesized to supply accessible hemoglobin was evaluated within an alternative meal (AM) containing whey powder and PBS for rearing the yellow fever mosquito . LC-MS/MS proteomics, microbial assays, and particle reduction techniques confirmed and characterized the functionality of hemoglobin in SDPB, while engorgement, fecundity, egg viability, and meal stability bioassays assessed AM performance.

Underwater endoscopic mucosal resection of adenomas and colorectal serrated lesions: a prospective clinical study.

Background: Underwater endoscopic mucosal resection (UEMR) without submucosal injection has been described as an alternative technique to the endoscopic resection of adenomas and colorectal serrated lesions. We aimed to assess the feasibility, safety, and efficacy of UEMR in a Brazilian setting.

Methods: This was a prospective observational study of consecutive patients who underwent UEMR between January and July 2019, in a single tertiary care center.

Influence of resin matrix on the rheology, translucency, and curing potential of experimental flowable composites for bulk-fill applications.

Objective: To propose monomer formulations that show an optimal degree of conversion as a function of depth for bulk-fill applications.

Methods: Four resin blends were formulated with methacrylate-based monomers: BisGMA + TEGDMA (control); BisEMA + BisGMA + TEGDMA (BisEMA-based); UDMA + BisGMA + TEGDMA (UDMA-based) and BisEMA + UDMA + BisGMA + TEGDMA (BisEMA + UDMA-based). For each material, a photoinitiating system and silanized filler particles were added.

Beauveria bassiana Culturing and Harvesting for Bioassays With House Flies.

The entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae) has been widely studied against a wide range of arthropod pests, including many of medical and veterinary importance. New investigators must sort through a wide array of published methods for the production, harvest, storage, and bioassay methods for this pathogen. Simplified methods for production of conidia using Sabouraud dextrose agar with yeast (SDYA) plates and two conidial harvesting methods are described.

Effect of soil cadmium on root organic acid secretion by forage crops.

The two forage species used in New Zealand pastoral agricultural systems, chicory (Cichorium intybus) and plantain (Plantago lanceolata) show differential ability to absorb and translocate cadmium (Cd) from roots to shoots. Chicory can accumulate Cd from even low Cd soils to levels that might exceed regulatory guidelines for Cd in fodder crops and food. Chicory and plantain were grown in soil-filled rhizocolumns under increasing Cd levels (0 (Control), 0.

Comparative Quantification of Trail-Following Behavior in Pest Ants.

A comparison of trail-following movement parameters of six major urban pest ants, (Forel) (Hymenoptera: Formicidae), , (Mayr), Buren, (Forel), and (Smith) demonstrated several differences in velocity of movement, amplitude of the deviations from a marked trail, percent fidelity to the trail, number of curves per unit of trail, and trail-following accuracy. and had the largest deviations from the marked trails and moved three times faster (25-30 mm/s) along the trail than the slowest ant, (< 10 mm/s), with other ants following between these extremes. Species differences in relation to going toward or returning from food were observed in a few cases, especially with for which velocity, amplitude, and trail fidelity differed between the foraging and return trails.

Sticky Trap Design Considerations for Entrapping Bed Bugs.

Little evidence has been presented on the usefulness of sticky traps for monitoring bed bugs, . We examined how the surface roughness around the adhesive of a sticky trap affects both bed bug behavior and adhesive entrapment. In the first assay, bed bugs were placed onto acetate paper discs with different roughness averages (R).

Mosquitocidal Chips Containing the Insect Growth Regulator Pyriproxyfen for Control of (Diptera: Culicidae).

were exposed to water treated with mosquitocidal chips containing the insecticide pyriproxyfen in a polymer formulation. Chips were tested under different conditions; different water volumes, in containers made of different material, and in water with different levels of organic matter. Treated chips caused 100% mortality of during their pupal stage independent of size or type of container, and the mount of organic matter contained in the water to which the mosquito larvae were exposed.

A Low-Cost, Passive Release Device for the Surveillance and Control of Mosquitoes.

Mosquitoes continue to be a major threat to global health, and the ability to reliably monitor, catch, and kill mosquitoes via passive traps is of great importance. Global, low-cost, and easy-to-use outdoor devices are needed to augment existing efforts in mosquito control that combat the spread of disease, such as Zika. Thus, we have developed a modular, portable, non-powered (passive), self-contained, and field-deployable device suitable for releasing volatiles with a wide range of applications such as attracting, repelling, and killing mosquitoes.

Factors Affecting Short-Range Host-Seeking for the Yellow Fever Mosquito (Diptera: Culicidae).

Understanding short-range cues (e.g., host odorants, heat, moisture) of host-seeking female Aedes aegypti L.

Laboratory Evaluation of a Novel Lethal Ovitrap for Control of Aedes aegypti.

Aedes aegypti (L.) (Diptera: Culicidae) are known to flourish in a variety of natural and residential habitats and are competent vectors of at least 22 different arboviruses, including dengue, chikungunya, and Zika. Their global distribution, anthropophilic nature, and vector competency make them species of interest for control.

Spatio-Temporal Distribution of Vector-Host Contact (VHC) Ratios and Ecological Niche Modeling of the West Nile Virus Mosquito Vector, Culex quinquefasciatus, in the City of New Orleans, LA, USA.

The consistent sporadic transmission of West Nile Virus (WNV) in the city of New Orleans justifies the need for distribution risk maps highlighting human risk of mosquito bites. We modeled the influence of biophysical and socioeconomic metrics on the spatio-temporal distributions of presence/vector-host contact (VHC) ratios of WNV vector, , within their flight range Biophysical and socioeconomic data were extracted within 5-km buffer radii around sampling localities of gravid female . The spatio-temporal correlations between VHC data and 33 variables, including climate, land use-land cover (LULC), socioeconomic, and land surface terrain were analyzed using stepwise linear regression models (RM).

Field evaluation of a semi-automatic funnel trap targeted the medically important non-biting flies.

Bait-trapping is a useful approach for monitoring fly population dynamics, and it is an effective tool for physical control of pest species. The aim of this study was to test a newly developed semi-automatic funnel fly trap with some modifications of the former prototype fly trap to study medically important fly population density. The efficacy of the semi-automatic funnel trap was assessed by field sampling during July 2013-June 2014 using 1-day tainted beef offal as bait.

Assessment of energy expenditure in individuals with post-poliomyelitis syndrome.

Methods: The Baecke questionnaire for the evaluation of habitual physical activity (HPA), assessment of quality of life (WHOQOL-Bref), and the Fatigue Severity Scale were administered to patients with PPS, poliomyelitis sequelae (PS) and to a control group (CG). Participated in the study 116 individuals (PPS=52,PS= 28,CG=36).

Results: Patients with PPS tended to increase their HPA from 10 to 20 years of age, compared with those in the PS group and the CG.

Morphology of immature stages of flesh flies, Boettcherisca nathani and Lioproctia pattoni (Diptera: Sarcophagidae).

The flesh flies are medically-important because the larvae found in the human corpses can provide evidence in forensic investigations through larva identification and their developmental rate. Firstly, we thoroughly described the larval morphology of Boettcherisca nathani and Lioproctia pattoni, using scanning electron microscopy (SEM). The third instar of the two species differed markedly in two characters: (1) spines between the prothorax and mesothorax-B.

Ecological niche modeling of mosquito vectors of West Nile virus in St. John's County, Florida, USA.

Background: The lack of available vaccines and consistent sporadic transmission of WNV justify the need for mosquito vector control and prediction of their geographic distribution. However, the distribution of WNV transmission is dependent on the mosquito vector and the ecological requirements, which vary from one place to another.

Methods: Presence/density data of two WNV mosquito vectors, Culex nigripalpus and Cx.

Behavioral Responses of Nymph and Adult Cimex lectularius (Hemiptera: Cimicidae) to Colored Harborages.

Behavioral bioassays were conducted to determine whether bed bug adults and nymphs prefer specific colored harborages. Two-choice and seven-choice behavioral color assays indicate that red (28.5%) and black (23.

Evaluation of Cyantraniliprole and Other Commercial Fly Baits under Laboratory and Field Conditions.

Laboratory and field trials were performed to evaluate the attractiveness and efficacy of commercial baits (cyantraniliprole; methomyl + (Z)-9-tricosene; dinotefuran + (Z)-9-tricosene; imidacloprid granular + (Z)-9-tricosene; and imidacloprid liquid + (Z)-9-tricosene). In choice tests; flies were most attracted to cyantraniliprole bait > dinotefuran + (Z)-9 > methomyl + (Z)-9 bait > imidacloprid granular + (Z)-9 bait > imidacloprid liquid + (Z)-9 bait. Significant degradation in bait efficacy was observed after two weeks of aging excluding imidacloprid granular; which began to degrade in field conditions after one week.

The Influence of Roughness and Pyrethroid Formulations on Bed Bug (Cimex lectularius L.) Resting Preferences.

Two-choice tests were conducted to examine the effect of surface roughness on the resting preference of bed bugs, Cimex lectularius L., on copper, basswood, and acrylic materials. The influence of pyrethroid formulation applications on resting preferences was also evaluated.

Nontarget effects of aerial mosquito adulticiding with water-based unsynergized pyrethroids on honey bees and other beneficial insects in an agricultural ecosystem of north Greece.

We assessed the nontarget effects of ultra-low-volume (ULV) aerial adulticiding with two new water-based, unsynergized pyrethroid formulations, Aqua-K-Othrine (FFAST antievaporant technology, 2% deltamethrin) and Pesguard S102 (10% d-phenothrin). A helicopter with GPS navigation technology was used. One application rate was tested per formulation that corresponded to 1.

[The workings of a Darwinist brain: Guedes Cabral and the evolutionism ofFunções do cérebro(1876)].

The book Funções do cérebro (Workings of the brain), by physician Domingos Guedes Cabral, is recognized as one of the first Brazilian works to defend Darwinist theories. In light of the need for more studies on the reception of evolutionism in Brazil, the article investigates this book in an effort to reveal how Guedes Cabral used evolutionism in his argumentation and to reveal the purpose of his arguments within a historical context. Findings indicate that Guedes Cabral relied on evolutionism as part of both an ideological project and an incipient eugenic theory, later developed in the early twentieth century.

Droplet sampling of an oil-based and two water-based antievaporant ultra-low volume insecticide formulations using Teflon- and magnesium oxide-coated slides.

We estimated the diameters below which 50% and 90% of the volume of droplets exist (Dv50 and Dv90, respectively) of 1 oil-based (Permanone 30-30) and 2 water-based (AquaReslin, Aqua-K-Othrine) antievaporant aerosols (with the Film Forming Aqueous Spray Technology [FFAST]) using Teflon- and magnesium oxide (MgO)-coated slides and determined whether the aging of the droplets on the slides (up to 60 min) exhibited any significant effect on Dv50 and Dv90 calculations. There were no significant differences in either Dv50 or Dv90 estimates on MgO-coated slides at 0 min and 60 min for all 3 products tested. On Teflon-coated slides, the only product that showed significant difference between 0 min and 60 min in both Dv50 and Dv90 estimates was Aqua-K-Othrine, perhaps due to a difference in formulation components.

Frequency and clinical manifestations of post-poliomyelitis syndrome in a Brazilian tertiary care center.

Objective: To determine the frequency and clinical manifestations of patients with post-poliomyelitis syndrome (PPS) in a Brazilian division of neuromuscular disorders.

Methods: A total of 167 patients with prior history of paralytic poliomyelitis was investigated for PPS, based on international diagnostic criteria. Other variables analyzed were: gender, race, age at poliomyelitis infection, age at PPS onset, and PPS symptoms.

Efficacy of aerial ultra-low volume applications of two novel water-based formulations of unsynergized pyrethroids against riceland mosquitoes in Greece.

We assessed the efficacy of ultra-low volume aerial adulticiding with 2 new water-based, unsynergized formulations of Aqua-K-Othrin (2% deltamethrin) and Pesguard S102 (10% d-phenothrin) against the riceland mosquitoes of Greece. A helicopter with Global Positioning System (GPS) navigation, real-time weather recording, and spray dispersal modeling (AgDISP) was utilized to accurately treat the experimental blocks by adjusting spray line positions to changing meteorological conditions. Two application rates were applied per formulation that corresponded to 0.