Prevalence and Clinical Profile of Human Salivirus in Children with Acute Gastroenteritis in Northern Italy, 2014-2015.

Objective: Human Salivirus (SalV) has been associated with gastroenteritis on all continents.

Methods: This paper presents the real-time RT-PCR assay for the detection of SalV in clinical fecal samples collected from 192 hospitalized children with acute gastroenteritis in Piedmont, Italy.

Results: The most commonly detected virus was Norovirus genogroup II (GII) (33.

Evaluation of two RNA extraction methods for human Parechovirus detection on pediatric stool specimens.

Background: Human Parechovirus (HPeVs), along with human enteroviruses is associated with gastrointestinal and respiratory infections. The aim of this study was to assess the performance characteristics of two nucleic acid extraction for HPeV-RNA quantitation, the RNAlev Extraction Kit associated with Maxwell automated nucleic acid extractor (Promega, Milan, Italy) and RNAzol manually protocol.

Methods: A total of 137 fecal specimens previously routinely screened for Rotavirus and Adenovirus were tested for HPeV virus.