Mathematical modelling reveals unexpected inheritance and variability patterns of cell cycle parameters in mammalian cells.

The cell cycle is the fundamental process of cell populations, it is regulated by environmental cues and by intracellular checkpoints. Cell cycle variability in clonal cell population is caused by stochastic processes such as random partitioning of cellular components to progeny cells at division and random interactions among biomolecules in cells. One of the important biological questions is how the dynamics at the cell cycle scale, which is related to family dependencies between the cell and its descendants, affects cell population behavior in the long-run.

Mutation, drift and selection in single-driver hematologic malignancy: Example of secondary myelodysplastic syndrome following treatment of inherited neutropenia.

Cancer development is driven by series of events involving mutations, which may become fixed in a tumor via genetic drift and selection. This process usually includes a limited number of driver (advantageous) mutations and a greater number of passenger (neutral or mildly deleterious) mutations. We focus on a real-world leukemia model evolving on the background of a germline mutation.

Dynamic cross talk model of the epithelial innate immune response to double-stranded RNA stimulation: coordinated dynamics emerging from cell-level noise.

We present an integrated dynamical cross-talk model of the epithelial innate immune response (IIR) incorporating RIG-I and TLR3 as the two major pattern recognition receptors (PRR) converging on the RelA and IRF3 transcriptional effectors. bioPN simulations reproduce biologically relevant gene-and protein abundance measurements in response to time course, gene silencing and dose-response perturbations both at the population and single cell level. Our computational predictions suggest that RelA and IRF3 are under auto- and cross-regulation.

Influence of gender on age of treatment with TKA and functional outcome.

Background: Previous studies suggest differences may exist between men and women in terms of knee function before and after total knee replacement. This may be related to the efficacy of the procedure itself or to differences in the severity of disability of male and female patients at the time of surgery.

Questions/purposes: We evaluated differences in the age, preoperative deformity, range-of-motion, and Knee Society scores of men and women who underwent TKA.

Implant durability and knee function after total knee arthroplasty in the morbidly obese patient.

This study investigated the effect of body mass index (BMI) on outcomes after cemented tricompartmental total knee arthroplasty (TKA). Functional and radiographic Knee Society scores in 71 patients (94 knees) with BMI 30 to 39 and 31 patients (41 knees) with BMI > or =40 were compared with 67 patients (85 knees) with BMI 20 to 29 at a mean follow-up of 5.4 years.

Posterior cruciate ligament substitution is not essential for excellent postoperative outcomes in total knee arthroplasty.

The study compared postoperative range of motion (ROM) and functional outcome of total knee arthroplasty (TKA) with a posterior cruciate ligament (PCL)-substituting knee prosthesis compared with an ultracongruent PCL-sacrificing design. Two hundred nine patients underwent primary TKA. Posterior stabilized design (121) and highly conforming, PCL-sacrificing, ultracongruent design (88) TKAs were reviewed.

The PCL significantly affects the functional outcome of total knee arthroplasty.

This study tests the hypothesis that patients receiving a posterior cruciate ligament (PCL)-retaining prosthesis have no difference in functional outcome compared to those receiving a cruciate-sacrificing, posterior-stabilized (PS) design. Forty-nine patients underwent a total knee arthroplasty (TKA), performed by a single surgeon using the same implant design with either a PCL-retaining or a PS tibial insert. Each patient completed a self-administered, validated Total Knee Function Questionnaire as well as the SF-36.

Mechanical alignment of tibial stems in revision total knee arthroplasty.

This multicenter, retrospective study evaluates the radiographic results of achieving optimal tibial alignment in revision total knee arthroplasty (TKA) using a single modular CoCr cemented or cementless stemmed implant design. Stem size and length also were evaluated. The hundred ninety-nine revision TKAs were performed between January 1993 and January 1996 by 13 experienced revision knee surgeons.