Publications by authors named "Roberta Fritz-Klaus"
Malignant ascites is a common clinical problem in ovarian cancer. NK cells are present in the ascites, but their antitumor activity is inhibited. The underlying mechanisms of the inhibition have yet to be fully elucidated.
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- - MUC16, a molecule linked to ovarian cancer (CA125), attaches in low amounts to immune cells via Siglec-9, but traditional imaging methods can't detect this effectively.
- - A new "digital" optical cytometry technique was developed, using plasmonic nanoparticles and deep learning, to analyze CA125 binding at nearly single-molecule sensitivity in blood immune cells from ovarian cancer patients.
- - Results showed that ovarian cancer patients had significantly higher CA125 levels on specific immune cell types compared to healthy donors, but there was no link between CA125 levels on immune cells and serum, indicating different roles for these measurements in cancer diagnostics.
Developing a mass spectrometry-based assay for the ovarian cancer biomarker CA125 (MUC16) is a desirable goal, because it may enable detection of molecular regions that are not recognized by antibodies and are therefore analytically silent in the current immunoassay. Additionally, the ability to characterize the CA125 proteoforms expressed by individuals may offer clinical insight. Enrichment of CA125 from malignant ascites may provide a high-quality source of this important ovarian cancer biomarker, but a reliable strategy for such enrichment is currently lacking.
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