Publications by authors named "Robert W Hartley"

We have identified Sti, the gene of a ribonuclease inhibitor from Saccharopolyspora erythraea, by using a T7 phage display system. A specific phage has been isolated from a genome library by a biopanning procedure, using RNase Sa3, a ribonuclease from Streptomyces aureofaciens, as bait. Sti, a protein of 121 amino acid residues, with molecular mass 13059 Da, is a homolog of barstar and other microbial ribonuclease inhibitors.

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Article Synopsis
  • The study used differential scanning calorimetry to analyze how protein complexes, specifically barnase, binase, and their inhibitor barstar, denature thermodynamically with varying free energy from -8 to -16 kcal/mol.
  • The research focused on both the wild-type proteins and their mutant versions, exploring the stability of these protein complexes during thermal unfolding.
  • Results supported the existing thermodynamics model by Brandts and Lin, which is designed for studying tight protein complexes that exhibit two-state thermal unfolding behavior.
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