Vascular streak dieback: A novel threat to redbud and other woody ornamental production in the United States.

Eastern redbud ( L.) is a popular and high-value woody ornamental plant native to the eastern and south-central United States of America (U.S.

In Vitro Regeneration from Leaf Explants of , a Critically Endangered Sunflower.

(Asteraceae), a whorled sunflower, is a perennial species restricted to a few locations in the southeastern United States and is now considered endangered. Therefore, restoring and protecting as a species is a priority. This study introduces a highly efficient in vitro adventitious plant regeneration system from leaf explants, utilizing five diverse specimens of , each representing distinct genotypes with phenotypic variations in leaf and stem morphology.

Signatures of prescribed fire in the microbial communities of are largely undetectable five months post-fire.

Prescribed burn is a management tool that influences the physical structure and composition of forest plant communities and their associated microorganisms. Plant-associated microorganisms aid in host plant disease tolerance and increase nutrient availability. The effects of prescribed burn on microorganisms associated with native ecologically and economically important tree species, such as L.

: An Updated Review of Conservation Efforts for an Endangered Plant.

(Small) Small, Ruth's golden aster, is an endangered Asteraceae species that grows in the riparian zone along small sections of two rivers in the Southern Appalachian Mountains of the United States of America (USA). Since 1985, the species has been listed under the Endangered Species Act by the United States Fish and Wildlife Service (USFWS). The mission of the USFWS is to conserve, protect, and enhance fish, wildlife, and plants and their habitats for the continued benefit of the American people.

Chemical profile and analysis of biosynthetic pathways and genes of volatile terpenes in Pityopsis ruthii, a rare and endangered flowering plant.

It is critical to gather biological information about rare and endangered plants to incorporate into conservation efforts. The secondary metabolism of Pityopsis ruthii, an endangered flowering plant that only occurs along limited sections of two rivers (Ocoee and Hiwassee) in Tennessee, USA was studied. Our long-term goal is to understand the mechanisms behind P.

A set of SSR markers to characterize genetic diversity in all Viburnum species.

About 160 species are classified within the Viburnum genus and many of these are cultivated for horticultural purposes. The vast dispersal of Viburnum makes the genus a useful model for studying evolutionary history and inferring how species expanded into their current distributions. Simple sequence repeat (SSR) markers were previously developed for five Viburnum species that were classified within the four major clades (Laminotinus, Crenotinus, Valvatotinus, and Porphyrotinus).

A novel genome sequence of Jasminum sambac helps uncover the molecular mechanism underlying the accumulation of jasmonates.

Jasminum sambac is a well-known plant for its attractive and exceptional fragrance, the flowers of which are used to produce scented tea. Jasmonate (JA), an important plant hormone was first identified in Jasminum species. Jasmine plants contain abundant JA naturally, of which the molecular mechanisms of synthesis and accumulation are not clearly understood.

Poa annua: An annual species?

As the Latin name annua implies, the species Poa annua L. is thought to have an annual life cycle. Yet, there are many reports in literature of P.

First report of leaf anthracnose on the Whorled Sunflower, Helianthus verticillatus, caused by Colletotrichum fioriniae in the United States.

Whorled sunflower, Helianthus verticillatus Small, is an endangered (U.S. Fish and Wildlife Service 2014) perennial sunflower species indigenous to the southern United States (Matthews et al.

Development and Characterization of 20 Genomic SSR Markers for Ornamental Cultivars of .

(Caprifoliaceae) is a genus of ornamental plants popular for its phenotypic variation and hardiness, that includes species hybridized to produce the commercially available cultivars. Despite its popularity, limited genetic resources exist for the genus. Twenty genomic simple sequence repeat (gSSR) markers distributed across the genome were developed using low coverage whole-genome sequencing data of Spilled Wine.

Chloroplast genome of the invasive Pyrus calleryana underscores the high molecular diversity of the species.

The complete chloroplast genome of Pyrus calleryana (GenBank OM541581.1) was developed by de novo assembly from whole-genome sequencing data. Reference-guided (P.

Microsatellite Loci Reveal High Genetic Diversity, Mutation, and Migration Rates as Invasion Drivers of Callery Pear () in the Southeastern United States.

Decne. (Callery pear) is a deciduous tree native to China, Japan, Korea, and Taiwan. It is a popular ornamental tree in the United States (US) with early spring blooms and vibrant fall color.

Genetic diversity in North American Cercis Canadensis reveals an ancient population bottleneck that originated after the last glacial maximum.

Understanding of the present-day genetic diversity, population structure, and evolutionary history of tree species can inform resource management and conservation activities, including response to pressures presented by a changing climate. Cercis canadensis (Eastern Redbud) is an economically valuable understory tree species native to the United States (U.S.

Identification of two Eleusine indica (goosegrass) biotypes of cool-season turfgrass resistant to dithiopyr.

Background: Turfgrass managers reported poor Eleusine indica control following applications of the mitosis-inhibiting herbicide dithiopyr in cool-season turfgrass. Field, glasshouse, and laboratory experiments were conducted to understand the response of these biotypes to dithiopyr and prodiamine.

Results: In field experiments at two locations with putative dithiopyr-resistant E.

Propagation Methods for the Conservation and Preservation of the Endangered Whorled Sunflower ().

Small, the whorled sunflower, is a perennial species only found at a few sites in the southeastern United States and was declared federally (USA) endangered in 2014. The species spreads locally via rhizomes and can produce copious seeds when sexually compatible genotypes are present. Vegetative propagation of the species via cuttings and the optimum conditions for seed germination have not been determined.

"Jumping Jack": Genomic Microsatellites Underscore the Distinctiveness of Closely Related and and Provide New Insights Into Their Evolutionary Past.

Downy mildews caused by obligate biotrophic oomycetes result in severe crop losses worldwide. Among these pathogens, and , two closely related oomycetes, adversely affect cucurbits and hop, respectively. Discordant hypotheses concerning their taxonomic relationships have been proposed based on host-pathogen interactions and specificity evidence and gene sequences of a few individuals, but population genetics evidence supporting these scenarios is missing.

Microsatellite Loci Reveal Genetic Diversity of Asian Callery Pear () in the Species Native Range and in the North American Cultivars.

Decne. (Callery pear) includes cultivars that in the United States are popular ornamentals in commercial and residential landscapes. Last few decades, this species has increasingly naturalized across portions of the eastern and southern US.

Microsatellite Markers from , the Cause of Blue Mold of Tobacco, Reveal Species Origin, Population Structure, and High Gene Flow.

is an obligate parasite that causes blue mold of tobacco. The pathogen reproduces primarily by sporangia, whereas the sexual oospores are rarely observed. A collection of 122 isolates of was genotyped using nine microsatellites to assess the population structure of individuals from subpopulations collected from central, southern, and western Europe; the Middle East; Central America; North America; and Australia.

Development and Characterization of 15 Novel Genomic SSRs for .

The genus is of particular interest to horticulturalists, phylogeneticists, and biogeographers. Despite its popularity, there are few existing molecular markers to investigate genetic diversity in this large genus, which includes over 160 species. There are also few polymorphic molecular tools that can delineate closely related species within the genus.

Conventional Gel Electrophoresis and TaqMan Probes Enable Rapid Confirmation of Thousand Cankers Disease from Diagnostic Samples.

Thousand cankers disease (TCD) is caused by the fungal pathogen and vectored by the walnut twig beetle . In infected walnut and butternut ( spp.) hosts and wingnut species (.

Complete chloroplast genome comparisons for Pityopsis (Asteraceae).

Pityopsis includes several regionally and one federally endangered species of herbaceous perennials. Four species are highly localized, including the federally endangered P. ruthii.

The ancient wave of polyploidization events in flowering plants and their facilitated adaptation to environmental stress.

Flowering plants, or angiosperms, consist of more than 300,000 species, far more than any other land plant lineages. The accumulated evidence indicates that multiple ancient polyploidy events occurred around 100 to 120 million years ago during the Cretaceous and drove the early diversification of four major clades of angiosperms: gamma whole-genome triplication in the common ancestor of core eudicots, tau whole-genome duplication during the early diversification of monocots, lambda whole-genome duplication during the early diversification of magnoliids, and pi whole-genome duplication in the Nymphaeales lineage. These four polyploidy events have played essential roles in the adaptive evolution and diversification of major clades of flowering plants.

Species diversity and phylogeography of (Asian dogwood) captured by genomic and genic microsatellites.

(Asian dogwood), an East Asia native tree, is the most economically important species of the dogwood genus, owing to its desirable horticultural traits and ability to hybridize with North America-native dogwoods. To assess the species genetic diversity and to better inform the ongoing and future breeding efforts, we assembled an herbarium and arboretum collection of 131 noncultivated specimens. Genotyping and capillary electrophoresis analyses of our collection with the newly developed genic and published nuclear genomic microsatellites permitted assessment of genetic diversity and evolutionary history of the species.

In Vitro Propagation of an Endangered by Axillary Bud Proliferation.

(Asteraceae), whorled sunflower, is a perennial species restricted to a few locations in the Southeastern United States. Habitat loss has caused to become rare, and since 2014, it has been federally listed as an endangered species. As a part of the recovery plan for the restoration and protection of , an efficient micropropagation protocol based on axillary shoot proliferation was developed.