Publications by authors named "Robert Soo Hoo"
Article Synopsis
- The study aimed to investigate differences in enzyme activities and oxidative damage markers among various liver mitochondrial sub-populations in mice, focusing on the effects of calorie restriction.
- Male C57BL/6J mice were divided into control and calorie-restricted groups after a month on a non-purified diet, and analyses were conducted after two months on a semi-purified diet to assess mitochondrial function.
- Key findings reveal that enzyme activities and oxidative damage markers varied across mitochondrial sub-populations, with calorie restriction showing specific benefits in the M1 and M3 fractions, suggesting a need for careful isolation techniques in mitochondrial studies.
Calorie restriction (CR) has been shown to decrease H(2)O(2) production in liver mitochondria, although it is not known if this is due to uniform changes in all mitochondria or changes in particular mitochondrial sub-populations. To address this issue, liver mitochondria from control and CR mice were fractionated using differential centrifugation at 1,000 g, 3,000 g and 10,000 g into distinct populations labeled as M1, M3 and M10, respectively. Mitochondrial protein levels, respiration and H(2)O(2) production were measured in each fraction.
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