Publications by authors named "Robert Ondrejka"

Article Synopsis
  • The study explored the antioxidative effects of α-tocopherol and β-carotene in squalene adjuvant emulsions used with a rabies vaccine, focusing on stability and effectiveness.
  • Findings showed that while α-tocopherol acted as a prooxidant, β-carotene successfully reduced oxidative injury and maintained emulsion stability even during prolonged homogenization.
  • The vaccine formulated with β-carotene led to a slower but consistent increase in rabies antibody levels, ultimately achieving a higher protective antibody concentration compared to the vaccine without β-carotene.
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Model experiments on laboratory animals (guinea pigs) were carried out to test the possible allergic reaction (possibility of sensitisation) to the repeated administration of an experimental lipoid adjuvant prepared on the basis of squalene (experimental squalene adjuvant--ESA). No significant differences were observed between the animals sensitised-provoked with ESA and control animals. In order to evaluate the local tissue reactivity (local reactogenity), also with regard to the process dynamics to the administration of ESA, comparative patho-anatomical and patho-histological examinations of tissues were carried out in the location of adjuvant administration.

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The authors prepared experimental lipoid adjuvant of the oil-in-water (O/W) type, based on squalene (ESA: experimental squalene adjuvant), intended for liquid inactivated veterinary vaccines. The final concentration of squalene in the vaccine was 5% (w/v). Detergents Poloxamer 105 in the concentration of 4% (w/v) and Abil-Care in the concentration of 2% were used as emulsifiers.

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