Randomized investigation of increased dialyzer membrane hydrophilicity on hemocompatibility and performance.

Background: Hemodialyzers should efficiently eliminate small and middle molecular uremic toxins and possess exceptional hemocompatibility to improve well-being of patients with end-stage kidney disease. However, performance and hemocompatibility get compromised during treatment due to adsorption of plasma proteins to the dialyzer membrane. Increased membrane hydrophilicity reduces protein adsorption to the membrane and was implemented in the novel FX CorAL dialyzer.

Micro-aerobic production of isobutanol with engineered .

KT2440 is emerging as a promising microbial host for biotechnological industry due to its broad range of substrate affinity and resilience to physicochemical stresses. Its natural tolerance towards aromatics and solvents qualifies this versatile microbe as promising candidate to produce next generation biofuels such as isobutanol. In this study, we scaled-up the production of isobutanol with from shake flask to fed-batch cultivation in a 30 L bioreactor.

Engineering KT2440 for the production of isobutanol.

We engineered for the production of isobutanol from glucose by preventing product and precursor degradation, inactivation of the soluble transhydrogenase SthA, overexpression of the native and genes, and implementation of the feedback-resistant acetolactate synthase AlsS from , ketoacid decarboxylase KivD from , and aldehyde dehydrogenase YqhD from . The resulting strain Iso2 produced isobutanol with a substrate specific product yield ( ) of 22 ± 2 mg per gram of glucose under aerobic conditions. Furthermore, we identified the ketoacid decarboxylase from to be a suitable alternative for isobutanol production, since replacement of from in Iso2 by the variant from yielded an identical Y.

Cell-free protein synthesis from non-growing, stressed Escherichia coli.

Cell-free protein synthesis is a versatile protein production system. Performance of the protein synthesis depends on highly active cytoplasmic extracts. Extracts from E.

High Substrate Uptake Rates Empower Vibrio natriegens as Production Host for Industrial Biotechnology.

The productivity of industrial fermentation processes is essentially limited by the biomass-specific substrate consumption rate ( ) of the applied microbial production system. Since depends on the growth rate (μ), we highlight the potential of the fastest-growing nonpathogenic bacterium, , as a novel candidate for future biotechnological processes. grows rapidly in BHIN complex medium with a μ of up to 4.

Site-Specific Cleavage of Ribosomal RNA in Escherichia coli-Based Cell-Free Protein Synthesis Systems.

Cell-free protein synthesis, which mimics the biological protein production system, allows rapid expression of proteins without the need to maintain a viable cell. Nevertheless, cell-free protein expression relies on active in vivo translation machinery including ribosomes and translation factors. Here, we examined the integrity of the protein synthesis machinery, namely the functionality of ribosomes, during (i) the cell-free extract preparation and (ii) the performance of in vitro protein synthesis by analyzing crucial components involved in translation.