Double-bundle anterior cruciate ligament reconstruction using autologous hamstrings with LARS augmentation demonstrates comparable outcomes to hamstrings alone, without evidence of synovitis or early osteoarthritis.

Purpose: To compare the clinical and radiological outcomes in patients undergoing anterior cruciate ligament reconstruction (ACLR) with, or without, LARS augmentation.

Methods: One-hundred and thirty-six patients that underwent double-bundle ACLR with (DB Hams/LARS, n = 67), or without (DB Hams, n = 69), LARS augmentation, were assessed clinically and with Magnetic Resonance Imaging (MRI) at a minimum of 7-years post-surgery. Patients were assessed via patient-reported outcome measures (PROMs), KT-1000 (laxity), isokinetic knee extensor and flexor strength and a 4-hop test battery.

A comparison of XRFS and ICP-OES methods for soil trace metal analyses in a mining impacted agricultural watershed.

Field portable X-ray fluorescence spectroscopy (XRFS) has become increasingly prevalent for in situ detection of trace metals, as it is both rapid and cost effective. The accuracy of in situ XRFS analyses has been questioned due to possible interferences from elevated soil moisture and organic content. In this study, three metal analysis protocols (Cd, Pb, Zn) were compared for surface soil samples collected near the Tar Creek Superfund Site in northeastern Oklahoma.

Metals retention in a net alkaline mine drainage impacted stream due to the colonization of the North American Beaver (Castor canadensis).

This study investigated two aspects of Castor canadensis (North American Beaver) colonization on a net alkaline mine drainage impacted tributary to Tar Creek (Ottawa County, OK, USA). Specifically, the retention of metals due to the presence of beaver dams, and metals contamination of sediments due to long-term loading, were examined. The Unnamed Tributary (UT) has been impacted by two mine drainage sources since at least 1979, with Cd, Fe, Pb, and Zn being the primary metals of concern.

Characterization of trace metal removal products in vertical flow bioreactor substrates at the Mayer Ranch Passive Treatment System in the Tar Creek Superfund Site.

A passive treatment system (PTS), including two parallel vertical flow bioreactors (VFBR), was constructed in 2008 for the treatment of unabated net-alkaline ferruginous mine drainage in the Tar Creek Superfund Site in northeastern Oklahoma. Water quality data collected since the PTS began operation indicate significant removal of trace metals in the VFBR. Results of a sequential extraction procedure (SEP) performed on substrate samples showed that the majority of Cd, Co, Fe, Ni, Pb, and Zn were retained in the refractory organic/sulfide fraction.

A Midterm Evaluation of Postoperative Platelet-Rich Plasma Injections on Arthroscopic Supraspinatus Repair: A Randomized Controlled Trial.

Background: Platelet-rich plasma (PRP) has been applied as an adjunct to rotator cuff repair to improve tendon-bone healing and potentially reduce the incidence of subsequent tendon retears.

Purpose: To investigate whether the midterm clinical and radiographic outcomes of arthroscopic supraspinatus repair are enhanced after repeated postoperative applications of PRP.

Study Design: Randomized controlled trial; Level of evidence, 1.

Synergistic negative effects of small-sized benthivorous fish and nitrogen loading on the growth of submerged macrophytes - Relevance for shallow lake restoration.

Rapid recruitment of small fish after biomanipulation in warm lakes may delay the reestablishment of submerged macrophytes, not least at high nutrient concentrations. Success has recently been obtained in controlling phosphorus (P) loading to many lakes, but nitrogen (N) inputs often remain high. To determine the interactive effects of N loading and the abundance of small-sized fish on the growth of the submerged macrophyte Vallisneria natans, we conducted an outdoor mesocosm experiment with a factorial design on the north shore of Lake Taihu, China.

Metal-contaminated potato crops and potential human health risk in Bolivian mining highlands.

This study assessed metals in irrigation water, soil and potato crops impacted by mining discharges, as well as potential human health risk in the high desert near the historic mining center of Potosí, Bolivia. Metal concentrations were compared with international concentration limit guidelines. In addition, an ingested average daily dose and minimum risk level were used to determine the hazard quotient from potato consumption for adults and children.

MDM2/CDK4 gene amplification in large/deep-seated 'lipomas': incidence, predictors and clinical significance.

This study of 140 cases assessed the incidence of MDM2/CDK4 gene amplification in lipomatous neoplasms with histological features of a lipoma but which were of clinical concern due to large size (≥50 mm) and/or deep-seated (subfascial) location. Univariate and multivariate statistical analyses were used to identify clinical, radiological and pathological predictors of gene amplification. Differences in local recurrence rates between amplified and non-amplified cases were assessed using survival analysis.

Evaluation of a universal flow-through model for predicting and designing phosphorus removal structures.

Phosphorus (P) removal structures have been shown to decrease dissolved P loss from agricultural and urban areas which may reduce the threat of eutrophication. In order to design or quantify performance of these structures, the relationship between discrete and cumulative removal with cumulative P loading must be determined, either by individual flow-through experiments or model prediction. A model was previously developed for predicting P removal with P sorption materials (PSMs) under flow-through conditions, as a function of inflow P concentration, retention time (RT), and PSM characteristics.

Mushroom tyrosinase: A model system to combine experimental investigation of enzyme-catalyzed reactions, data handling using R, and enzyme-inhibitor structural studies.

The activity of mushroom tyrosinase can be measured by monitoring the conversion of phenolic compounds into quinone derivatives using spectrophotometry. This article describes a series of experiments which characterize the functional properties of tyrosinase, the analysis of the resulting data using R to determine the kinetic parameters, and the exploration of the structural properties of tyrosinase-inhibitor complexes. Tyrosinase assay development and subsequent activity measurements, in the presence of varying pH, substrate concentration and inhibitors, offers the opportunity to learn the enzyme characterization skills relevant to a research laboratory setting.

Incidence, degree, and development of graft hypertrophy 24 months after matrix-induced autologous chondrocyte implantation: association with clinical outcomes.

Background: Graft hypertrophy is a common occurrence after periosteal, collagen-covered and matrix-induced autologous chondrocyte implantation (MACI).

Purpose/hypothesis: The purpose of this study was to investigate the incidence, development, and degree of graft hypertrophy at 24 months after MACI. The hypothesis was that graft hypertrophy would not be associated with clinical outcome at 24 months.

Removal of Less Commonly Addressed Metals via Passive Cotreatment.

The viability of removing less commonly addressed metals (e.g., Cd, Cu, Ni, and Pb) in a passive cotreatment concept was tested using a microcosm-scale, three-stage batch reactor system in which acid mine drainage from an abandoned adit on Cerro Rico de Potosí and raw municipal wastewater from Potosí, Bolivia, were introduced at a 5:1 ratio.

Translational research will fail without surgical leadership: SCOTRRCC a successful surgeon-led Nationwide translational research infrastructure in renal cancer.

Background: High quality human biosamples with associated high quality clinical data are essential for successful translational research. Despite this, the traditional approach is for the surgeon to act as a technician in the tissue collection act. Biomarker research presents multiple challenges and the field is littered with failures.

Reliability of a 3 T MRI protocol for objective grading of supraspinatus tendonosis and partial thickness tears.

Background: Partial thickness supraspinatus tears and tendonosis can be managed either nonoperatively or by various arthroscopic techniques. New biologic treatment approaches are currently being investigated. MRI is commonly used for objective imaging outcome evaluation but there is a lack of reliability studies.

Anatomic repair of the distal biceps tendon cannot be consistently performed through a classic single-incision suture anchor technique.

Background: Distal biceps tendon ruptures commonly occur in active men, and surgical repair through a single-incision technique using suture anchors has become common. The current study assessed whether an anatomic repair of the biceps to the radial tuberosity can be consistently achieved through a single-incision technique.

Methods: Acute distal biceps tendon repairs using the single-incision technique were retrospectively reviewed.

Current perspectives on percutaneous vertebroplasty: current evidence/controversies, patient selection and assessment, and technique and complications.

Osteoporotic-associated vertebral compression fractures are a major public health concern, dwarfing even hip fractures in incidence in the United States. These fractures carry a significant morbidity and mortality burden and also represent a major growing source of consumption of scarce heath resources. Percutaneous vertebroplasty remains a commonly used and safe technique for the symptomatic treatment of vertebral compression fractures, both osteoporotic- and neoplastic-induced.

Novel passive co-treatment of acid mine drainage and municipal wastewater.

A laboratory-scale, four-stage continuous-flow reactor system was constructed to test the viability of high-strength acid mine drainage (AMD) and municipal wastewater (MWW) passive co-treatment. Synthetic AMD of pH 2.6 and acidity of 1870 mg L(-1) as CaCO3 equivalent containing a mean 46, 0.

Fluidized bed ash and passive treatment reduce the adverse effects of acid mine drainage on aquatic organisms.

Elevated concentrations of acidity and metals in acid mine drainage (AMD) may be effectively addressed by active and passive treatment technologies. However, typical evaluations consider only chemical water quality with little if any regard for biological metrics. Robust evaluations including both chemical and biological indicators of water quality improvement are needed.

The effects of a dredge excavation pit on benthic macrofauna in offshore Louisiana.

Over two years after the original creation of a sand excavation pit 8 km off the Louisiana coast, benthic macrofauna communities and sedimentary characteristics are still effected. Macrofaunal communities inside the pit had lower abundance, biomass, and diversity than communities outside the pit. This difference, however, was only significant with some of the stations outside the pit.

Testicular microlithiasis: prevalence and risk of concurrent and interval development of testicular tumor in a referred population.

Aims: To identify prevalence of testicular microlithiasis on ultrasound in a referred population and risk of concurrent and interval testicular tumor development.

Methods: Retrospective review of our radiology database revealed 4363 scrotal ultrasounds were performed over a six-year period. Ultrasound findings were correlated with our hospital pathological database.

Temporal and spatial development of surface soil conditions at two created riverine marshes.

The amount of time it takes for created wetlands to develop soils comparable to natural wetlands is relatively unknown. Surface soil changes over time were evaluated in two created wetlands (approximately 1 ha each) at the Olentangy River Wetland Research Park in Columbus, Ohio. The two wetlands were constructed in 1993 to be identical in size and geomorphology, and maintained to have the same hydrology.