Mixed method analysis of anthropogenic groundwater contamination of drinking water sources in Malawi.

Groundwater contamination poses significant challenges to public health and sustainable development in Malawi, where approximately 80 % of the population relies on groundwater sources for drinking water. This study investigates the presence and drivers of nitrate and E. coli contamination in groundwater used for drinking.

Spatial model of groundwater contamination risks from pit-latrines in a low-income country.

Pit-latrines are central to achieving UN Sustainable Development Goal 6 (SDG 6) of ensuring "clean water and sanitation for all". Unless safely managed, pit-latrines result in groundwater contamination, which increases morbidity and mortality. Despite this, there have been no long-term spatial projections of future pit-latrine contamination risks.

The Status of Sanitation in Malawi: Is SDG6.2 Achievable?

Ensuring access to adequate and equitable sanitation and ending open defecation by 2030 is the focus of Sustainable Development Goal 6.2 (SDG6.2).

Acute health risks to community hand-pumped groundwater supplies following Cyclone Idai flooding.

This longitudinal flood-relief study assessed the impact of the March 2019 Cyclone Idai flood event on E. coli contamination of hand-pumped boreholes in Mulanje District, Malawi. It established the microbiological water-quality safety of 279 community supplies over three phases, each comprising water-quality survey, rehabilitation and treatment verification monitoring.

Fluoride occurrence in the lower East African Rift System, Southern Malawi.

Countries located on the East African Rift System (EARS) are vulnerable to fluoride in their groundwater; a vulnerability for the developing country of Malawi at the southern rift periphery that is not well characterised. Groundwater fluoride occurrence in Malawi is documented here to better understand and manage fluoride risks posed. Available literature and Gov't of Malawi archive fluoride data spanning some fifty years have been collated and augmented by our own 2016-18 surveys of groundwater quality in Southern Malawi, targeting deep-sourced springs.

A comprehensive optimum integrated water resources management approach for multidisciplinary water resources management problems.

A holistic Integrated Water Resources Management (IWRM) model can be difficult to implement and the associated high-dimension optimization problems' complexity often forces the decision makers to downscale such problems. These challenges however have motivated this research to develop a comprehensive Optimum IWRM approach (OP-IWRM) using a many-objective optimization algorithm to solve complex and large-scale problems. The approach employs the social, economic, and environmental objectives; ground and surface water resources; and water infrastructure for river basin management to: (1) improve the relevant revenues, (2) enhance community welfare, and (3) pave the road for the decision makers to set better investment policy.

Responding to salinity in a rural African alluvial valley aquifer system: To boldly go beyond the world of hand-pumped groundwater supply?

Effective response to groundwater salinity in the developing world may critically safeguard drinking-water supplies. Groundwater resources throughout rural Africa are exploited by a vast and increasing number of hand-pumped boreholes for community supply. Our research in TA Ngabu (Shire Valley), Southern Malawi aims to: define groundwater-salinity problem occurrence within the semi-arid alluvial-valley aquifer, rural developing-world setting; critique current capacity to respond; and, to discuss future response options - in particular considering the need to explore alternative options that boldly go beyond the world of hand-pumped groundwater supply.

Performance of a field-scale biological permeable reactive barrier for in-situ remediation of nitrate-contaminated groundwater.

We report the performance of a field-scale permeable reactive barrier (PRB) for the biological treatment of nitrate-contaminated groundwater. The reactive material of the PRB consisted of a mixture of gravel and mulch as a carbon source for denitrifying bacteria. The PRB was equipped with a delivery system that allowed injecting NO at controlled rates from the surface directly into the up-gradient layer of the PRB.

Optimum socio-environmental flows approach for reservoir operation strategy using many-objectives evolutionary optimization algorithm.

Water resource system complexity, high-dimension modelling difficulty and computational efficiency challenges often limit decision makers' strategies to combine environmental flow objectives (e.g. water quality, ecosystem) with social flow objectives (e.

Local scale water-food nexus: Use of borehole-garden permaculture to realise the full potential of rural water supplies in Malawi.

Local-scale opportunities to address challenges of the water-food nexus in the developing world need to be embraced. Borehole-garden permaculture is advocated as one such opportunity that involves the sustainable use of groundwater spilt at hand-pump operated borehole supplies that is otherwise wasted. Spilt water may also pose health risks when accumulating as a stagnant pond.

Risk assessment to groundwater of pit latrine rural sanitation policy in developing country settings.

Parallel global rise in pit-latrine sanitation and groundwater-supply provision is of concern due to the frequent spatial proximity of these activities. Study of such an area in Malawi has allowed understanding of risks posed to groundwater from the recent implementation of a typical developing-country pit-latrine sanitation policy to be gained. This has assisted the development of a risk-assessment framework approach pragmatic to regulatory-practitioner management of this issue.

Comprehensive database of Manufactured Gas Plant tars. Part C. Heterocyclic and hydroxylated polycyclic aromatic hydrocarbons.

Rationale: Coal tars are a mixture of organic and inorganic compounds that were by-products from the manufactured gas and coke making industries. The tar compositions varied depending on many factors such as the temperature of production and the type of retort used. For this reason a comprehensive database of the compounds found in different tar types is of value to understand both how their compositions differ and what potential chemical hazards are present.

Comprehensive database of Manufactured Gas Plant tars. Part B. Aliphatic and aromatic compounds.

Rationale: Coal tars are a mixture of organic and inorganic compounds that were produced as a by-product from the manufactured gas and coke making industries. The composition of the tar produced varies depending on many factors; these include the temperature of production and the type of retort used. As different production processes produce different tars, a comprehensive database of the compounds present within coal tars from different production processes is a valuable resource.

Comprehensive database of Manufactured Gas Plant tars. Part A. Database.

Rationale: Coal tars are a mixture of organic and inorganic compounds that were by-products from the manufactured gas and coke making industries. Different manufacturing processes have resulted in the production of distinctly different tar compositions. This study presents a comprehensive database of compounds produced using two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GCxGC/TOFMS), analysing 16 tar samples produced by five distinct production processes.

Comprehensive composition of Creosote using comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GCxGC-TOFMS).

Creosote is a distillation product of coal tar and is widely used as wood preservative for railway sleepers, utility poles and for other applications. Creosote can have potentially negative effects on the environment and many of the components are toxic. This study presents the analysis of a Creosote sample from a former wood impregnation plant located in the UK.

Frequency-dependent ultrasound-induced transformation in E. coli.

Ultrasound-enhanced gene transfer (UEGT) is continuing to gain interest across many disciplines; however, very few studies investigate UEGT efficiency across a range of frequencies. Using a variable frequency generator, UEGT was tested in E. coli at six ultrasonic frequencies.

Environmental and toenail metals concentrations in copper mining and non mining communities in Zambia.

Copper mining contributes to increased concentrations of metals in the environment, thereby increasing the risk of metals exposure to populations living in and around mining areas. This study investigated environmental and toenail metals concentrations of non-occupational human exposure to metals in 39 copper-mining town residents and 47 non-mining town residents in Zambia. Elevated environmental concentrations were found in samples collected from the mining town residents.

A culture-independent approach to unravel uncultured bacteria and functional genes in a complex microbial community.

Most microorganisms in nature are uncultured with unknown functionality. Sequence-based metagenomics alone answers 'who/what are there?' but not 'what are they doing and who is doing it and how?'. Function-based metagenomics reveals gene function but is usually limited by the specificity and sensitivity of screening strategies, especially the identification of clones whose functional gene expression has no distinguishable activity or phenotypes.

Multivariate statistical methods for the environmental forensic classification of coal tars from former manufactured gas plants.

Compositional disparity within a set of 23 coal tar samples (obtained from 15 different former manufactured gas plants) was compared and related to differences between historical on-site manufacturing processes. Samples were prepared using accelerated solvent extraction prior to analysis by two-dimensional gas chromatography coupled to time-of-flight mass spectrometry. A suite of statistical techniques, including univariate analysis, hierarchical cluster analysis, two-dimensional cluster analysis, and principal component analysis (PCA), were investigated to determine the optimal method for source identification of coal tars.

Carbon isotopic fractionation of CFCs during abiotic and biotic degradation.

Carbon stable isotope ((13)C) fractionation in chlorofluorocarbon (CFC) compounds arising from abiotic (chemical) degradation using zero-valent iron (ZVI) and biotic (landfill gas attenuation) processes is investigated. Batch tests (at 25 °C) for CFC-113 and CFC-11 using ZVI show quantitative degradation of CFC-113 to HCFC-123a and CFC-1113 following pseudo-first-order kinetics corresponding to a half-life (τ(1/2)) of 20.5 h, and a ZVI surface-area normalized rate constant (k(SA)) of -(9.

Ultra resolution chemical fingerprinting of dense non-aqueous phase liquids from manufactured gas plants by reversed phase comprehensive two-dimensional gas chromatography.

Ultra resolution chemical fingerprinting of dense non-aqueous phase liquids (DNAPLs) from former manufactured gas plants (FMGPs) was investigated using comprehensive two-dimensional gas chromatography coupled with time of flight mass spectrometry (GC×GC TOFMS). Reversed phase GC×GC (i.e.

Simplifying and improving the extraction of nitrate from freshwater for stable isotope analyses.

Determining the isotopic composition of nitrate (NO(3)(-)) in water can prove useful to identify NO(3)(-) sources and to understand its dynamics in aquatic systems. Among the procedures available, the 'ion-exchange resin method' involves extracting NO(3)(-) from freshwater and converting it into solid silver nitrate (AgNO(3)), which is then analysed for (15)N/(14)N and (18)O/(16)O ratios. This study describes a simplified methodology where water was not pre-treated to remove dissolved organic carbon (DOC) or barium cations (added to precipitate O-bearing contaminants), which suited samples with high NO(3)(-) (≥ 00 μM or 25 mg L(-1) NO(3)(-)) and low DOC (typically < 17 μM of C or 5 mg L(-1) C) levels.

Development of an advanced on-line position-specific stable carbon isotope system and application to methyl tert-butyl ether.

We present an advanced system for on-line position-specific carbon isotope analysis. The main limitation of on-line intramolecular isotope ratio measurements has been that optimal pyrolytic fragments are obtained mostly at temperatures where the analyte has not completely reacted. As a result of undetermined isotopic fractionation, the isotopic signatures of the pyrolysis products are not strictly equal to these of the equivalent moieties in the parent molecule.

Resolving genetic functions within microbial populations: in situ analyses using rRNA and mRNA stable isotope probing coupled with single-cell raman-fluorescence in situ hybridization.

Prokaryotes represent one-half of the living biomass on Earth, with the vast majority remaining elusive to culture and study within the laboratory. As a result, we lack a basic understanding of the functions that many species perform in the natural world. To address this issue, we developed complementary population and single-cell stable isotope ((13)C)-linked analyses to determine microbial identity and function in situ.

Late-surviving megafauna in Tasmania, Australia, implicate human involvement in their extinction.

Establishing the cause of past extinctions is critical if we are to understand better what might trigger future occurrences and how to prevent them. The mechanisms of continental late Pleistocene megafaunal extinction, however, are still fiercely contested. Potential factors contributing to their demise include climatic change, human impact, or some combination.