Publications by authors named "Robert M Johnston"
Article Synopsis
- A disulfide-bridged peptide drug candidate consists of two oligopeptide chains with 11 and 12 amino acids, linked by a disulfide bond at one end.
- A biotechnology process was developed to efficiently produce these peptides by separately creating each oligopeptide chain in E. coli, then combining them through cleavage and chemical reactions.
- A novel heterodimeric system using a leucine zipper was designed to enhance the formation of the disulfide bond, employing computer modeling to optimize amino acid interactions and resulting in a successful synthesis of the target peptide.
