Effects of nonylphenol and ethinylestradiol on copper redhorse (Moxostoma hubbsi), an endangered species.

The copper redhorse, Moxostoma hubbsi, is an endangered species endemic to Quebec. The presence of contaminants, in particular endocrine disrupting chemicals (EDCs), in its habitat has been advanced as partly responsible for the reproductive difficulties encountered by the species. In the present study, immature copper redhorse were exposed to the estrogenic surfactant nonylphenol (NP; 1, 10 and 50µg/l) and the synthetic estrogen 17α-ethinylestradiol (EE2; 10ng/l) for 21 days in a flow-through system.

Purification and partial characterization of vitellogenin from spotted wolffish (Anarhichas minor) and development of an enzyme-linked immunosorbent assay for the determination of gender and sexual maturity.

Vitellogenin (VTG) from spotted wolffish, Anarhichas minor, a candidate species for cold-water marine aquaculture, was purified by MgCl₂/EDTA precipitation followed by a two-step chromatographic procedure. VTG had an apparent molecular mass of 470 kDa, as determined by gel filtration, and an amino acid composition similar to those of other teleosts. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of the purified VTG revealed a major band with a relative molecular weight of 166 kDa and some minor bands.

Effects of chronic exposures to the herbicides atrazine and glyphosate to larvae of the threespine stickleback (Gasterosteus aculeatus).

Atrazine (ATR) and glyphosate (GLY) are among the most widely used herbicides in Canada, yet there is relatively little information concerning their toxicity to early life stages of marine fish. The threespine stickleback (Gasterosteus aculeatus) reproduces in coastal habitats which receive runoff of pesticides during the summer, the peak season of herbicide use. Sticklebacks have biomarkers for effects of both estrogenic and androgenic contaminants.

Hybrid ELISAs for vitellogenins of the endangered copper redhorse Moxostoma hubbsi and the shorthead redhorse Moxostoma macrolepidotum (Cypriniformes, catostomidae).

The shorthead redhorse (sr) Moxostoma macrolepidotum is endemic in North America, while in Canada the copper redhorse (cr) Moxostoma hubbsi is classified as endangered. A commercial heterologous carp vitellogenin (VTG) sandwich ELISA underestimates VTGs in these species. Hybrid ELISAs, using anti-carp VTG antibodies and purified sr- and cr-VTGs as standards, were validated to measure VTGs in plasma and surface mucus of redhorse species.

A lateral flow immunoassay for rapid evaluation of vitellogenin levels in plasma and surface mucus of the copper redhorse (Moxostoma hubbsi).

We tested a lateral flow immunoassay (LFIA) for detecting vitellogenin (VTG) in plasma and surface mucus of copper redhorse, Moxostoma hubbsi, a threatened fish species. The LFIA detected VTG in samples from estradiol-induced fish, though there was no reaction in samples from noninduced individuals. The minimum detection range was 0.

Purification of vitellogenin from smooth flounder (Pleuronectes putnami) and measurement in plasma by homologous ELISA.

Male smooth flounder (Pleuronectes putnami) were induced to produce vitellogenin (VTG) by injection of 17beta-estradiol (E2). Anion exchange chromatography of precipitated plasma from E2-injected resulted in a single peak consisting of VTG. Smooth flounder VTG has an approximate molecular mass of approximately 520 kDa, determined by gel filtration with molecular weight standards.