Development and Optimization of AAV hFIX Particles by Transient Transfection in an iCELLis(®) Fixed-Bed Bioreactor.

Adeno-associated virus (AAV) vectors are increasingly popular in gene therapy because they are unassociated with human disease, replication dependent, and less immunogenic than other viral vectors and can infect a variety of cell types. These vectors have been used in over 130 clinical trials, and one AAV product has been approved for treatment of lipoprotein lipase deficiency in Europe. To meet the demand for the increasing quantities of AAV required for clinical trials and treatment, a scalable high-capacity technology is required.

Investigation of retentive force reduction and wear of the locator attachment system with different implant angulations.

Purpose: To evaluate the effect of cyclic disengagement on the retentive force and wear patterns of pairs of three Locator inserts (blue, pink, and clear) in vitro.

Materials And Methods: Implant analogs (Astra Tech) were positioned into aluminum blocks parallel to each other and perpendicular to the horizontal plane, with one analog angulated mesially by 10 degrees (0/10), or with two implant analogs each angulated mesially by 5 degrees (5/5). Ninety Locator attachments, in 15 pairs of each standard retention strength (blue, pink, and clear), underwent 2,500 cycles of testing, lubricated with artificial saliva.

Wheat germ agglutinin as a counterstain for immunofluorescence studies of equine hoof lamellae.

Equine laminitis is a common, painful, debilitating condition of the hoof that is a leading cause of disability in horses, often necessitating euthanasia. The equine hoof represents an extreme evolutionary adaptation of an epidermal structure homologous to the human or murine nail units. Immunohistochemistry is frequently utilized in the study of the pathophysiology of laminitis.

Three-dimensional processing deformation of three denture base materials.

Statement Of Problem: Heat-polymerized polymethyl methacrylate denture bases deform during and after polymerization, and this deformation may affect the clinical performance of complete dentures.

Purpose: The purpose of this study was to investigate the processing deformation of 3 denture base materials on a standardized anatomic model by using a contact scanner and surface matching software.

Material And Methods: Maxillary reproductions of a definitive cast were digitized by using a contact scanner.

Effect of two different disinfectants on dimensional stability of newer alginate impression materials over five days.

The effect of two different disinfectants on the dimensional stability of two alginate impression materials over five days was investigated. 60 impressions were made under standardised conditions, 30 with each alginate, of which ten were disinfected in each disinfectant and ten were controls. Impressions were stored over a period of five days in a container with 100% humidity.

Immediate replacement complete dentures: pitfalls of ignoring traditional teaching and established practice.

A thirty-year-old female had immediate replacement dentures made by a general practitioner and her teeth extracted and the dentures fitted under general anaesthetic by her local oral surgeon three months previously. Anterior and posterior teeth had been extracted and no attempt had been made to smooth or shape the ridges. Both dentures were constructed with gum fitted anterior teeth without labial flanges.

Characterization of the porcine testis-expressed gene 11 (Tex11).

Testis expressed gene 11 () is essential for meiosis and male fertility in the mouse. Currently, little is known about the control of spermatogenesis in non-rodent animal models such as pigs. Here, we characterized the sequence and expression profile of the porcine gene.

An investigation into the effects of metal primer and surface topography on the tensile bond strength between cobalt chromium alloy and composite resin.

This study examined the influence of surface preparation and metal primer on the tensile bond strength between cobalt chromium alloy and composite resin. The bond strength between 168 cobalt chromium alloy dumb-bells with one of three test surfaces (beaded, machined or sandblasted) to composite resin were tested. Half of each group were treated with metal primer.

The fatigue life of a cobalt-chromium alloy after laser welding.

The aim of this study was to investigate the fatigue life of laser welded joints in a commercially available cast cobalt-chromium alloy. Twenty rod shaped specimens (40 mm x 1.5 mm) were cast and sand blasted.

Dimensional stability of newer alginate impression materials over seven days.

The aim of this study was to determine the dimensional stability of the so called '5 day stable' alginates. Ten specimens each of three alginate materials were prepared using a standardised impression technique and a typodont model. Travelling microscope measurements were recorded for six distances at 24 hour intervals.

Tensile strength of laser welded cobalt-chromium alloy with and without an argon atmosphere.

The tensile strength and depth of weld of two cobalt chromium alloys before and after laser welding with and without an argon gas atmosphere were investigated. Using two cobalt chromium alloys, rod shaped specimens (5 cm x 1.5 mm) were cast.

Is the bond between acrylic resin denture teeth and denture base resin stronger if they are both made by the same manufacturer?

A previous study suggested that a stronger bond may be achieved between acrylic resin denture base material and acrylic denture teeth when both are made by the same manufacturer. Three denture base acrylic resins from three different manufacturers were bonded to three different acrylic resin denture teeth, one of which was manufactured by each of the manufacturers of the base material. In each group there was a trend that the bond strength achieved between the teeth and base material from the same manufacturer was higher than the unmatched pairs but statistical significance was not achieved.

Effect of curing cycle on the tensile strength of the bond between heat cured denture base acrylic resin and acrylic resin denture teeth.

The effect of different curing cycles on the tensile strength of the bond between one brand of cross-linked acrylic resin teeth and three heat cured denture base acrylic resins was tested. There were differences in the tensile bond strength between the three heat cured denture base acrylic resins and the three curing cycles used. The bond strength of the acrylic resin denture base material made by the same manufacturer as the cross-linked acrylic resin denture teeth was higher.

Effect of GC METALPRIMER II on bond strength of heat-cured acrylic resin to titanium alloy (Ti-6Al-4V) with two different surface treatments.

This study investigated the bond strength of heat-polymerized acrylic resin to titanium alloy using a proprietary bonding agent (GC Metalprimer II). Two surface treatments (sandblasted or roughened with a tungsten carbide bur) were compared for their effect on bond strength with or without thermal cycling. Eighty specimens of heat-polymerized acrylic resin bonded to titanium alloy (Ti-6Al-4V) were prepared: 20 specimens (control) and 60 used a bonding agent.

Effect of surfactant on surface hardness of dental stone and investment casts produced from polyvinyl siloxane duplicating materials.

Polyvinylsiloxane duplicating materials are typically treated with a topical surfactant before pouring dental models, but the use of topical surfactants in the dental laboratory may affect the surface hardness of the resultant models. The effect of two different topical surfactants on surface hardness of two dental stones (FujiRock and Dentstone) and one phosphate bonded investment material (Croform WB) produced from polyvinyl siloxane (PVS) dental laboratory duplicating moulds was investigated. Topical surfactants affected the surface hardness of FujiRock, Dentstone and Croform WB investment material.

Long-term dimensional stability and reproduction of surface detail of four polyvinyl siloxane duplicating materials.

Objectives: Duplicating materials must routinely accurately reproduce the details of dental casts and thus contribute significantly to the close adaptation and success of a removable prosthesis or fixed indirect restoration. It is important to establish the long-term dimensional stability of polyvinyl siloxane materials (PVS) as they are widely used in dental technology and over the duration of a course of treatment, are stored under dental laboratory conditions. The dimensional stability of four PVS duplicating materials was investigated over a 13-week period.

Tensile bond strength of heat and self-cured acrylic denture base resins to the inner and outer layers of two-layered acrylic resin denture teeth.

The tensile bond strength of the inner and outer layers of two-layered acrylic resin denture teeth to heat cured and self-curing acrylic resin denture base material was tested. The bond between the heat-cured resin and the inner layer of the denture teeth was significantly stronger than the bond between the heat-cured resin and the outer layer of the denture teeth. The bond between both layers of the denture teeth and the self-curing acrylic resin was significantly weaker than with the heat-cured acrylic resin.

Dimensional change of heat-cured acrylic resin dentures with three different cooling regimes following a standard curing cycle.

The aim of the study was to compare dimensional changes in poly(methylmethacrylate) complete denture bases resulting from three different cooling regimens following a standard heating cycle. Changes in three separate dimensions were measured on ten dentures within each cooling regimen after curing, and before and after removing the denture from the cast using a computer imaging system. No consistent differences occurred as a result of removing the denture from the cast.

Chairside polishing of heat-cured acrylic resin: an SEM and EDA study.

Purpose: This study investigated the surfaces produced on two commonly used heat-cured acrylic resins by two chairside polishing systems compared to conventional lathe polishing. It used energy dispersive analysis (EDA) and scanning electron microscopy (SEM) to investigate the abrasive materials bonded in the silicone points that are marketed for chairside polishing of acrylic resin.

Materials And Methods: Using the three polishing systems (conventional as control, Shofu acrylic polishing system [AcryPoint], and Kenda Queen silicone polisher), three operators polished specimens using a defined protocol.

Events in the mould during heat processing of poly(methyl methacrylate).

Recordings of temperature and pressure in the mould have raised questions concerning the flow of poly(methylmethacrylate) during heat processing. Three simple experiments investigated flow of polymerised and partly polymerised poly(methylmethacrylate). Flow was not observed at normal processing temperatures.

Dimensional change in complete dentures fabricated by injection molding and microwave processing.

Statement Of Problem: Acrylic resin complete dentures undergo dimensional changes during polymerization. Techniques with injection molding and polymerization and microwave polymerization are reported to reduce these changes and thereby improve clinical fit. These dimensional changes need to be quantified.

Dimensional change in dentures processed in silicone and stone moulds.

Anecdotal evidence suggests that many dental laboratories are surrounding the teeth and gingival margins of the wax patterns for complete dentures with silicone laboratory putty prior to investing in dental stone or plaster to facilitate deflasking and polishing. This project investigates differences in dimension during processing when putty is used. Two groups of ten standardised dentures, one group with silicone, one group without were processed.