Immunological alterations during the clinical and recovery phases of experimental swine dysentery.

The aim of this study was to examine changes in the systemic immune response during the incubation period and following the onset of clinical swine dysentery, including the recovery period. Ten healthy conventional pigs were inoculated with Brachyspira hyodysenteriae. Blood was sampled at pre-inoculation, at days 4 and 14 post-inoculation, during the first 4 days with clinical signs of dysentery and at days 1, 3, 7, 11 and 15 of the recovery period.

Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology.

Background: Multiplex flow cytometry is in widespread use for detection of cytokines in human samples. However, no report on the measurement of porcine cytokines using this method has previously been published. We report on the detection of the porcine proinflammatory cytokines TNF-alpha, IL-8, and IL-1beta by the xMap-assay for multiplex flow cytometry.

Effect of exercise on proglycogen and macroglycogen content in skeletal muscles of pigs with the Rendement Napole mutation.

Objective: To investigate influence of the Rendement Napole (RN-) mutation on proglycogen (PG) and macroglycogen (MG) content in skeletal muscles before and after exercise and evaluate glycogen concentrations within various muscle fiber types.

Animals: 5 pigs with the RN- mutation and 3 noncarrier pigs.

Procedure: Pigs performed 2 exercise tests on a treadmill.

Differences in lymphocyte subpopulations and cell counts before and after experimentally induced swine dysentery.

The aim of this study was to examine the levels of circulating leukocytes and lymphocyte subpopulations before and immediately after experimentally induced swine dysentery. Twenty-one healthy crossbred pigs (approximately 22 kg) were orally inoculated with Brachyspira hyodysenteriae. Blood was sampled before inoculation and when clinical signs of swine dysentery occurred.