Use of GC clamps in DHPLC mutation scanning.

Objective: Development of a systematic mutation detection assay strategy for denaturing high performance liquid chromatography (DHPLC).

Design: Adaptation of Guanine and Cytosine (GC)-clamping from denaturing gradient gel electrophoresis (DGGE) to DHPLC.

Methods: Three target sequences harboring known allelic variants were studied to develop a general DHPLC assay design strategy.

Decreased transcription of the human FCGR2B gene mediated by the -343 G/C promoter polymorphism and association with systemic lupus erythematosus.

The role for inhibitory Fc gamma receptors class IIb (FcgammaRIIb) in the onset, progression and severity of several animal models of autoimmune diseases is well established. By contrast, the pathogenic potential of FcgammaRIIb in human autoimmune diseases remains largely unknown. Here we report the identification of a polymorphism in the human FCGR2B promoter (dbSNP no.