Amplification of proopiomelanocortin mRNA in canine skin: preliminary results.

Skin and pituitary specimens were obtained from a normal chow-chow cross-bred dog. Total messenger RNA (mRNA) was extracted, reversed transcribed and the proopiomelanocortin (POMC) mRNA was amplified by polymerase chain reaction using canine specific primers. The expected 391 bp amplification product was detected in both canine skin and pituitary samples.