Publications by Robert J Filkins

Publications by authors named "Robert J Filkins"

Appl Immunohistochem Mol Morphol

July 2016

A multiplex assay was developed to detect 8 breast cancer biomarkers on a single tissue slide using staining and imaging technologies from General Electric.
An unexpected membranous staining of Ki-67 was found to be an artifact resulting from the binding of two antibodies in the multiplexing process.
By blocking with rabbit serum, researchers established a successful method to multiplex the biomarkers without any antibody cross-reactivity.

Histopathology

January 2014

A new multiplexed immunofluorescence technology allows for detailed imaging of multiple breast cancer biomarkers on a single tissue sample, focusing on cellular interactions and co-expression.
The method involves an automated microfluidic flow cell for efficient staining and imaging, and has been tested on a small number of breast tumors to compare with traditional methods.
This approach shows consistent results with conventional immunohistochemistry and offers improved accuracy and the ability to visualize more biomarkers within the same tissue sample.

Proc Natl Acad Sci U S A

July 2013

Limitations in identifying unique proteins and DNA in tissue samples hinder our understanding of health and disease.
The multiplexed fluorescence microscopy method (MxIF) allows for detailed analysis of multiple biological markers in tissue samples by chemically inactivating fluorescent dyes after imaging to enable reuse.
MxIF demonstrates similar accuracy to traditional methods in identifying protein markers while revealing significant diversity in cancer cells, paving the way for advancements in research and diagnostics.

J Pathol Inform

November 2011

Accurate focusing in whole slide imaging is challenging due to the variability in tissue topography, making it difficult for traditional scanning systems to maintain focus while processing images quickly.
This review discusses the shortcomings of first-generation whole slide scanners and introduces a new method called independent dual sensor scanning, which uses continuous autofocus.
The new approach separates image acquisition from focusing, enabling faster scanning with better focus accuracy, and the paper also examines the pros and cons of this technique compared to traditional systems.

Opt Express

June 2008

A new autofocusing technique for digital microscopy uses only two intermediate images to efficiently focus on samples.
The method is designed to work with a standard commercial inverted microscope.
It automates the brightfield and fluorescence imaging processes for examining histopathology tissue sections.

Proc SPIE Int Soc Opt Eng

January 2008

Near-infrared (NIR) fluorescence can assist surgeons by providing real-time imaging during operations, improving the visibility of tissues.
The study presents a low-cost electronic circuit that can control LEDs for fluorescence measurements at frequencies up to 35 MHz, which helps enhance the quality of fluorescence images.
Details about the circuit design and components are made available online for others to access and utilize in related research.