optical recordings of ion dynamics in mouse corneal primary nociceptive terminals.

This protocol aims to measure ion dynamics in nociceptive terminal endings in intact mice . We describe viral injection of GCaMP6s + RFP into trigeminal ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive terminals that express GCaMP6s and RFP. This fast and high-resolution optical recording technique enables studying a nociceptive terminal's functional molecular network in physiological and pathological conditions.