Publications by authors named "Robert G Solomon"
Article Synopsis
- Site-directed PCR-based mutagenesis is commonly used to create mutations, but existing methods usually require DNA clones, which aren't always available.
- The article introduces an improved RT-PCR method that allows for the creation of multiple mutations directly from total RNA, focusing on the human beta-actin gene.
- The study found that by using specific RT-PCR conditions and three mutagenic primers, they produced seven different clones with a mix of single and multiple mutations, optimizing the process for future applications.
A (15)N-labelled peripheral-subunit binding domain (PSBD) of the dihydrolipoyl acetyltransferase (E2p) and the dimer of a solubilized interface domain (E3int) derived from the dihydrolipoyl dehydrogenase (E3) were used to investigate the basis of the interaction of E2p with E3 in the assembly of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus. Thirteen of the 55 amino acids in the PSBD show significant changes in either or both of the (15)N and (1)H amide chemical shifts when the PSBD forms a 1 : 1 complex with E3int. All of the 13 amino acids reside near the N-terminus of helix I of PSBD or in the loop region between helix II and helix III.
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