Publications by authors named "Robert F Donat"
resPAINT: Accelerating Volumetric Super-Resolution Localisation Microscopy by Active Control of Probe Emission.
Angew Chem Weinheim Bergstr Ger
October 2022
Points for accumulation in nanoscale topography (PAINT) allows practically unlimited measurements in localisation microscopy but is limited by background fluorescence at high probe concentrations, especially in volumetric imaging. We present reservoir-PAINT (resPAINT), which combines PAINT and active control of probe photophysics. In resPAINT, an activatable probe "reservoir" accumulates on target, enabling a 50-fold increase in localisation rate versus conventional PAINT, without compromising contrast.
View Article and Find Full Text PDFPoints for accumulation in nanoscale topography (PAINT) allows practically unlimited measurements in localisation microscopy but is limited by background fluorescence at high probe concentrations, especially in volumetric imaging. We present reservoir-PAINT (resPAINT), which combines PAINT and active control of probe photophysics. In resPAINT, an activatable probe "reservoir" accumulates on target, enabling a 50-fold increase in localisation rate versus conventional PAINT, without compromising contrast.
View Article and Find Full Text PDFUnderstanding mechanisms of antibody synergy is important for vaccine design and antibody cocktail development. Examples of synergy between antibodies are well-documented, but the mechanisms underlying these relationships often remain poorly understood. The leading blood-stage malaria vaccine candidate, CyRPA, is essential for invasion of Plasmodium falciparum into human erythrocytes.
View Article and Find Full Text PDFArticle Synopsis
- - The COVID-19 pandemic has caused significant health and economic challenges, and there are currently no approved treatments available.
- - Researchers have isolated an antibody called EY6A from a recovered COVID-19 patient, which has been shown to effectively neutralize both SARS-CoV-2 and SARS-CoV-1 by tightly binding to the viral spike protein.
- - Structural analyses indicate that EY6A targets a crucial region on the spike protein that helps stabilize it, suggesting that this antibody could be a promising therapeutic option for treating COVID-19.