Nuclease-Resistant c-di-AMP Derivatives That Differentially Recognize RNA and Protein Receptors.

The ability of bacteria to sense environmental cues and adapt is essential for their survival. The use of second-messenger signaling molecules to translate these cues into a physiological response is a common mechanism employed by bacteria. The second messenger 3'-5'-cyclic diadenosine monophosphate (c-di-AMP) has been linked to a diverse set of biological processes involved in maintaining cell viability and homeostasis, as well as pathogenicity.

RTD-1mimic containing γPNA scaffold exhibits broad-spectrum antibacterial activities.

Macrocyclic peptides with multiple disulfide cross-linkages, such as those produced by plants and those found in nonhuman primates, as components of the innate immunity, hold great promise for molecular therapy because of their broad biological activities and high chemical, thermal, and enzymatic stability. However, for some, because of their intricate spatial arrangement and elaborate interstrand cross-linkages, they are difficult to prepare de novo in large quantities and high purity, due to the nonselective nature of disulfide-bond formation. We show that the disulfide bridges of RTD-1, a member of the θ-defensin subfamily, could be replaced with noncovalent Watson-Crick hydrogen bonds without significantly affecting its biological activities.

Results and factors affecting outcome of revision surgery for shoulder instability.

We conducted a clinical study identifying the causes of failure and the variables affecting outcome in 28 patients with failed open or arthroscopic anterior shoulder reconstruction for anterior glenohumeral instability. All patients underwent an open revision stabilization procedure. Surgical outcomes at a minimum 24 months' follow-up were available in 25 patients.

The accuracy of distal posterior interosseous and anterior interosseous nerve injection.

Purpose: To standardize a technique of delivering a local anesthetic to the posterior interosseous nerve (PIN) and anterior interosseous nerve (AIN) by using the anatomic landmarks of the wrist and to evaluate the accuracy of the technique in a cadaver model.

Methods: Techniques for PIN and AIN injection and for PIN injection alone are described. Techniques were tested in a fresh frozen cadaver model by using methylene blue injections.

Adult acquired flat foot deformity: clinical and radiographic examination.

Symptomatic adult acquired flat foot deformity is encountered in the orthopedic office on a frequent basis. Although many causes exist, a careful history and a stepwise approach to the physical examination will clue the examiner into making the correct diagnosis and provide appropriate treatment. Radiographs serve as an adjunct and assist in verifying the examination findings.