Promoter optimisation of lentiviral vectors for efficient insulin gene expression in canine mesenchymal stromal cells: potential surrogate beta cells.

Background: The lack of an ideal cell type that can be easily acquired, modified to produce insulin, and re-implanted has been a limitation for ex vivo insulin gene therapy. Canine diabetes is currently treated with human insulin and is a good model for human diabetes. Mesenchymal stromal cells (MSCs) are a promising candidate cell type for gene therapy.

Unilateral squamous cell carcinoma of the renal pelvis with hydronephrosis in a cat.

A 4-year-old female neutered domestic shorthair cat was presented for evaluation of gradual onset of lethargy and anorexia. Physical examination revealed moderate abdominal distension. Investigations performed included complete blood count, serum biochemistry, urinalysis, pyelocentesis, abdominal fluid analysis, abdominal ultrasonography and exploratory celiotomy.

Trichobezoar obstruction after stapled jejunal anastomosis in a dog.

Objective: To describe an unusual long-term complication of circular end-to-end anastomosis (CEEA) stapling in a dog.

Study Design: Clinical case report.

Animal: An 11-year-old, female neutered, Labrador Retriever.