A Critical Review and Perspective of Honey in Tissue Engineering and Clinical Wound Healing.

Historically, honey has been regarded as a potent agent in bacterial inhibition and wound healing. An increased prevalence of antibiotic resistant pathogens spurred an initial resurgence in honey's clinical popularity, with it quickly finding a place in wound care and regenerative medicine. However, this renewed usage demanded a need for improved delivery and overall research of its bioactive properties.

Controlling formaldehyde exposures in an academic gross anatomy laboratory.

This report describes efforts over a more than a 15-year period to improve air quality and reduce exposures to formaldehyde during anatomical dissections at the Yale University School of Medicine, including first-year medical student gross anatomy classes. During this time, a number of steps were taken to improve general ventilation system efficiency and work practices in the original facility. Subsequently, during the design phase for a new research and teaching building, a new anatomical laboratory was planned to incorporate 42 individually ventilated dissection tables.

Fire safety recommendations for administration of isoflurane anesthesia in oxygen.

A researcher at the author's facility was carrying out a routine surgical procedure in a mouse that was anesthetized with vaporized isoflurane. When the researcher brought an active cauterizer close to the mouse, a flame erupted from the anesthesia nosecone. An investigation concluded that the fire was ignited when the cauterizer came into contact with the oxygen-enriched atmosphere that was streaming from the anesthetic equipment.

Containment capabilities of animal transfer stations.

Animal-transfer and cage-changing stations are portable downdraft-filtered clean benches that have been specifically modified for small-rodent handling and cage changing from two or more sides, and that are advertised by their manufacturers as providing improved laboratory animal allergen control. The authors evaluated the dust containment capability of three such devices under exaggerated challenge conditions, compared design features, and conclude that animal-transfer stations can be a useful addition to an institutional laboratory animal allergy control program.

An Exhaust Hood for Animal Perfusions.

Animal perfusion is a common technique in the physiology research laboratory and typically involves the use of aqueous formaldehyde. Depending on the specific procedure conducted, the animal studied, and availability and efficiency of local ventilation systems, these procedures may create occupational over-exposure to formaldehyde, a regulated carcinogen. We report the design and performance of a local exhaust hood specifically developed for primate and small mammal perfusion.