Publications by authors named "Rob Van Rossom"
Article Synopsis
- Human pluripotent stem cell-derived hepatocyte-like cells (HLCs) are promising for liver disease studies, drug testing, and toxicity evaluations but face challenges like high production costs and complex differentiation processes.
- An automated liquid handling system was used in the differentiation of HLCs, significantly reducing costs by about 79% and allowing for the screening of cheaper alternatives to growth factors.
- The study found that using a combination of small molecules and Laminin-521 produced HLCs with similar characteristics and functionality to those made with traditional growth factors, enabling a scalable and cost-effective method for HLC production.
Tagging of endogenous stress response genes can provide valuable in vitro models for chemical safety assessment. Here, we present the generation and application of a fluorescent human induced pluripotent stem cell (hiPSC) reporter line for Heme oxygenase-1 (HMOX1), which is considered a sensitive and reliable biomarker for the oxidative stress response. CRISPR/Cas9 technology was used to insert an enhanced green fluorescent protein (eGFP) at the C-terminal end of the endogenous HMOX1 gene.
View Article and Find Full Text PDFReprogramming can occur by the introduction of key transcription factors (TFs) as well as by epigenetic changes. We demonstrated that histone deacetylase inhibitor (HDACi) Trichostatin A (TSA) combined with a chromatin remodeling medium (CRM) induced expression of a number of definitive endoderm and early and late pancreatic marker genes. When CRM was omitted, endoderm/pancreatic marker genes were not induced.
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