Biological Assay-Guided Fractionation and Mass Spectrometry-Based Metabolite Profiling of L. Cytotoxic Compounds against Lung Cancer A549 Cell Line.

L. (Guyabano) leaves are reported to exhibit anticancer activity against cancer cells. In this study, the ethyl acetate extract from guyabano leaves was purified through column chromatography, and the cytotoxic effects of the semi-purified fractions were evaluated against A549 lung cancer cells using in vitro MTS cytotoxicity and scratch/wound healing assays.