Publications by authors named "Rita Zgheib"

The genus is composed of Gram-negative, fastidious, facultative intracellular bacteria that can cause bacteremia in mammals and various disorders in humans. Rodents have been reported as reservoirs of more than 30 species, seven of which cause zoonotic infections. In the present study, the isolation of sp.

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species are involved in various human diseases, causing a range of clinical manifestations; animals are considered as the main reservoirs, transmitting diverse species of through direct contact and haematophagous insects. Here, we characterize a new species, sp. nov.

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The connection between humans and digital technologies has been documented extensively in the past decades but needs to be evaluated through the current global pandemic. Artificial Intelligence(AI), with its two strands, Machine Learning (ML) and Semantic Reasoning, has proven to be a great solution to provide efficient ways to prevent, diagnose and limit the spread of COVID-19. IoT solutions have been widely proposed for COVID-19 disease monitoring, infection geolocation, and social applications.

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is the etiological agent of Q fever, a worldwide zoonosis able to cause large outbreaks. The disease is polymorphic. Symptomatic primary infection is named acute Q fever and is associated with hepatitis, pneumonia, fever, and auto-immune complications while persistent focalized infections, mainly endocarditis, and vascular infections, occur in a minority of patients but are potentially lethal.

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In 2018, Corynebacterium sanguinis strain Marseille-P8776 was isolated from the blood of a 64-year-old woman suffering from breast cancer who had undergone chemotherapy and radiotherapy. Following whole-genome sequencing, the chromosome of strain Marseille-P8776 was 2,613,836 bp long with a G+C content of 64.92%, 2,568 protein-coding genes, and 64 RNA genes.

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The Microbial Culturomics Project aiming to discover several bacterial species made it possible to isolate the strain Marseille-P4308 from a stool sample of a healthy indigenous Congolese volunteer. Strain Marseille-P4308 is a Gram-positive coccus shaped bacterium that optimally grows at 37 °C. The 16S rRNA gene sequence of the strain has a 96.

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The growing application of metagenomics to different ecological and microbiome niches in recent years has enhanced our knowledge of global microbial biodiversity. Among these abundant and widespread microbes, the candidate phyla radiation (CPR) group has been recognized as representing a large proportion of the microbial kingdom (>26%). CPR are characterized by their obligate symbiotic or exoparasitic activity with other microbial hosts, mainly bacteria.

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Strain Marseille-P9829 was isolated from a bone sample collected from an open right fibula fracture from a 46-years old patient. Strain Marseille-P9829 (= CSUR P9829 = DSM 110695) was a Gram-negative, non-spore-forming and non-motile bacterium. This strain had a positive catalase activity but was oxidase-negative.

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Strains Marseille-P2265 (=CSUR P2265 =DSM 102082) and Marseille-P3890 (=CSUR P3890 =CCUG 72341) were isolated from stool samples using the culturomics approach. The 16S rRNA gene sequences of both strains were sequenced and compared by BLASTn to the NCBI database. Strains Marseille-P2265 and Marseille-P3890 were most closely related to Acutalibacter muris with identities of 94.

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Article Synopsis
  • The study focused on antimicrobial resistance and usage trends at Saint George Hospital University Medical Center in Beirut during the SARS-CoV-2 pandemic, analyzing data from 2015 to 2020.
  • It found a decrease in the isolation density of blood stream infections (BSI) and drug-resistant infections during 2020, with significant drops in carbapenem-resistant and difficult-to-treat infections.
  • The report attributed these positive trends to effective antimicrobial stewardship and infection control measures, indicating that the pandemic did not worsen existing resistance issues.
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Bacillus velezensis, a species first described in 2005, has been mostly associated with plants and the environment. To date, there is no genome available for this species from human samples. In this announcement, we present the genome of Bacillus velezensis strain Marseille-Q1230, which was isolated from a stool sample from a child suffering from severe acute malnutrition.

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We present the genome sequences of two carbapenemase-producing sequence type 405 Escherichia coli clinical isolates, strains Marseille-Q1950 and Marseille-Q1951. The isolates were obtained 1 month apart during the patient's hospitalization in Lebanon, in May (Marseille-Q1950) and June (Marseille-Q1951) 2019. The genome sizes of strains Marseille-Q1950 and Marseille-Q1951 were 5,181,515 bp and 5,213,451 bp, respectively.

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A pan-drug-resistant Klebsiella pneumoniae strain was isolated from the blood of a 70-year-old critically ill patient in April 2019. Interestingly, the patient recovered and was discharged home a month later. The genome of strain Marseille-Q1949 is 5,607,584 bp long and has a 57.

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species are reemerging infectious agents that are transmitted by arthropod vectors among animals and/or humans. At least 13 of the 35 currently recognized species are pathogenic for humans. Most of the pathogenic species, except and are zoonotic agents with animal reservoirs, including cats, dogs, coyotes, foxes, cattle, and rodents.

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Living organisms interact with each other during their lifetime, leading to genomes rearrangement and sequences transfer. These well-known phenomena give these organisms mosaic genomes, which challenge their classification. Moreover, many findings occurred between the IXXth and XXIst century, especially the discovery of giant viruses and candidate phyla radiation (CPR).

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Five novel bacterial strains, Marseille-P1476 (=CSURP1476=DSM 100642), Marseille-P3256 (=CSURP3256=CECT 9977), Marseille-P2936 (=CSURP2936=DSM 103159), Marseille-P2912 (=CSURP2912=DSM 103345) and Marseille-P3197 (=CSURP3197=CCUG 71847), were isolated from various human specimens. These five strains were not identified at the species level by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Following 16S rRNA gene sequence comparisons with the GenBank database, the highest nucleotide sequence similarities of all studied strains were obtained to members of the paraphyletic genus .

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Strain Marseille-P1302 was isolated from the stool of a 2-year-old Nigerian boy suffering from Kwashiorkor, a form of severe acute malnutrition. The strain grows in aerobic atmosphere and bacterial cells are Gram-positive cocci ranging in diameter from 0.8 to 1 μm.

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was described as a new bacterial species, a contaminant isolated from a biopharmaceutical production process in Sweden. Since the genome sequence was not available, we performed draft genome sequencing. The genome of strain CCM 7991 (=CCUG 53591 = DSM 28178 = Marseille-Q4544) was 4,038,814 bp long, with a G+C content of 53.

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Thanks to the progress and decreasing costs in genome sequencing technologies, more than 250,000 bacterial genomes are currently available in public databases, covering most, if not all, of the major human-associated phylogenetic groups of these microorganisms, pathogenic or not. In addition, for many of them, sequences from several strains of a given species are available, thus enabling to evaluate their genetic diversity and study their evolution. In addition, the significant cost reduction of bacterial whole genome sequencing as well as the rapid increase in the number of available bacterial genomes have prompted the development of pangenomic software tools.

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In 2007, was first described as a bacterial isolate from a sea salt evaporation pond. As no genome sequence was previously available for this species, we performed whole-genome sequencing. The chromosome of strain Q1438 was 3,784,443 bp long with 36% G+C content, 3,830 protein-coding genes, and 74 RNA genes.

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In 2016, was reported as a bacterial species isolated from a healthy Peruvian male. In 2018, a clinical strain from the same species was isolated from the stool of a French patient with kidney cancer. The genome of this strain, P8546, was 1,725,465 bp long, with 33.

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