A catalogue of selected derivatization reactions to label molecules in the subnanomolar or nanomolar range for CE and micro-HPLC with LIF detection.

Sensitivity is a major obstacle to biochemists studying complex biological mixtures. Laser-induced fluorescence (LIF) is a very sensitive method that can be used with micro-HPLC (microHPLC) or capillary electrophoresis studies and provides an effective solution to these problems. Derivatization is a very useful tool in LIF detection because it allows for the detection of many compounds and is selective, i.

Choice of different dyes to label tyrosine and nitrotyrosine.

In this work, we will present some attempts to analyze tyrosine and nitrotyrosine using capillary electrophoresis and either UV-Visible detection or laser-induced fluorescence (LIF) detection. An argon ion (488 nm) laser is used for fluorescein isothiocyanate (FITC) and 7-fluoro-4-nitro-2,1,3-benzoxadiazole (NBD-F). A near infrared (780 nm) laser is used for NIR 780 derivatives.