Characterization of Polish feline B. henselae isolates by multiple-locus tandem repeat analysis and pulse-field gel electrophoresis.

Knowledge about molecular epidemiology of B. henselae is important for recognizing the geographical distribution of strains and identification of isolates virulent for humans. Eleven Polish feline B.

A new typing technique for the Rickettsiales Ehrlichia ruminantium: multiple-locus variable number tandem repeat analysis.

Ehrlichia ruminantium (ER) is a member of the order Rickettsiales transmitted by Amblyomma ticks. This obligatory intracellular bacterium is the causative agent of a fatal disease in ruminants, named heartwater. It represents a constraint on breeding development in sub-Saharan Africa and in the Caribbean.

Molecular epidemiology of feline and human Bartonella henselae isolates.

Multiple locus variable number tandem repeat analysis was performed on 178 Bartonella henselae isolates from 9 countries; 99 profiles were distributed into 2 groups. Human isolates/strains were placed into the second group. Genotype I and II isolates shared no common profile.

Development of discriminatory multiple-locus variable number tandem repeat analysis for Bartonella henselae.

Bartonella henselae is a zoonotic bacterium that infects cats and humans. Several attempts have been made to develop typing techniques for epidemiological purposes; however, most of the techniques developed do not appear to be sufficiently discriminatory or easy to use. In order to develop multilocus variable number tandem repeat (VNTR) analysis (MLVA) for B.