Pharmacokinetic properties of the antimuscarinic drug [3H]-hexahydro-sila-difenidol in the rat.

The pharmacokinetics of tritiated hexahydro-sila-difenidol [( 3H]-HHSiD) were examined in rats. Furthermore, the distribution of radioactivity was studied by means of whole body autoradiography. After i.

Histological analysis of ultrasonic images of the prostate: an accurate technique.

Ultrasonography of the prostate furnishes images which still cannot be fully interpreted morphologically. In a cadaver study, the ultrasound images of two groups (21 and 19) prostates, obtained in a water bath, were compared with histology slides taken at corresponding levels. In the first part of the study, using a 4 MHz probe, there was a correlation between hyperechoic lesions and stone formations in 9 out of 15 cases.

Whole body distribution of the quaternary ammonium compound thiazinamium (N-methylpromethazine) and promethazine in monkey and mice.

The distribution of 35S-ringlabeled thiazinamium (N-methyl-promethazine) methylsulphate (35S-Th.) and its tertiary analog 35S-promethazine hydrochloride (35S-Pr.) have been studied by means of whole body autoradiography in a squirrel monkey and in mice.

Whole-body autoradiography after systemic and topical administration of methyl acrylate in the guinea pig.

Whole-body autoradiography was performed in the guinea pig with methyl (2,3-14C)-acrylate. Radioactive material quickly disappeared from the body after oral and, somewhat slower, after i.p.

Cryosectioning of undecalcified tissues for immunofluorescence.

The present report describes a procedure for preparing 4--6 micrometers cryostat sections of undecalcified fresh frozen tissue which contain hard tissue, for immunofluorescence. The apparatus used is a cryomicrotome originally designed for cutting sections for whole body autoradiography. To obtain cryostat sections suitable for tissue immunofluorescence the standard procedure was modified with respect to the hardness and edges of the microtome knife, the temperature of the cryostat and the carboxymethyl cellulose concentration of the embedding material.