Publications by authors named "Riffat John"

Plants often experience variations in light intensity, referred to as light stress, that negatively impact important aspects of plant growth and development, including photosynthesis and antioxidant system. The photosynthetic machinery is susceptible to these disturbances, especially photosystem II and its reaction centers. We aimed to evaluate the role of brassinosteriod in plants under both high and low light conditions by examining various physiological parameters such as photosynthetic efficiency, pigment levels, and enzymatic activity of various antioxidant enzymes in one month old tomato plants.

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Article Synopsis
  • * The study examined the role of Si transporters in wheat genotypes with different resistance levels to stripe rust, revealing that resistant genotypes accumulated more Si than susceptible ones.
  • * Results showed that Si supplementation increased Si transporter expression and Si accumulation in resistant wheat, highlighting the importance of these mechanisms for improving plant defense against biotic stressors.
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Background Of The Study: Digitalis purpurea (L) is an important medicinal plant growing at Alpine region of Himalayas and withstands low temperatures and harsh climatic conditions existing at high altitude. It serves as an ideal plant system to decipher the tolerance to cold stress (CS) in plants from high altitudes.

Methods And Results: To understand the complexity of plant response to CS, we performed a comparative physiological and biochemical study complemented with proteomics in one-month-old D.

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Background: Low temperature (LT) stress is one of the major environmental stress factors affecting the growth and yield of maize (Zea mays L.). Hence, it is important to unravel the molecular mechanisms behind LT stress tolerance to improve molecular breeding in LT tolerant genotypes.

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The genetic modification of plants for the removal of inorganic pollutants from contaminated soil and water bodies is an emerging area for addressing environmental concerns. This approach is based on the ability of plants to take up and accumulate heavy metals, with efficiency being dependent on the underlying mechanisms of heavy metal accumulation and tolerance. A robust antioxidant pathway is determinantal for heavy metal uptake and accumulation and, therefore, in this study, we evaluated the transgenic tomato plants installed with Ascorbate Glutathione (ASA-GSH) pathway genes for uptake, accumulation, and response to mercury (Hg).

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Nanoparticles are immensely diverse both in terms of quality and sources of emission into the environment. Nowadays, nanotechnologies are developing and growing at a rapid pace without specific rules and regulations, leading to a severe effect on environment and affecting the labours in outdoor and indoor workplaces. The continue and enormous use of NPs for industrial and commercial purposes, has put a pressing need to think whether the increasing use of these NPs could overcome the severe environmental effects and unknown human health risks.

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Maize (Zea mays L.), a major multipurpose crop for food, feed and energy is extremely susceptible to environmental perturbations and setting off the major factors for limiting maize yield. Generally, plant yields are reduced and significantly lost to adverse environments and biotic strains.

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Legumes are the most important crop plants in agriculture, contributing 27% of the world's primary food production. However, productivity and production of Legumes is reduced due to increasing environmental stress. Hence, there is a pressing need to understand the molecular mechanism involved in stress response and legumes adaptation.

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Cold stress is considered as one of the major environmental factors that adversely affects the plant growth and distribution. Therefore, there arises an immediate need to cultivate effective strategies aimed at developing stress-tolerant crops that would boost the production and minimise the risks associated with cold stress. In this study, a novel cold-responsive protein1 (BoCRP1) isolated from Brassica oleracea was ectopically expressed in a cold susceptible tomato genotype Shalimar 1 and its function was investigated in response to chilling stress.

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Stacking Glutathione-Ascorbate pathway genes (PgSOD, PgAPX, PgGR, PgDHAR and PgMDHAR) under stress inducible promoter RD29A imparts significant tolerance to drought and salinity stress in Solanum lycopersicum. Although the exposure of plants to different environmental stresses results in overproduction of reactive oxygen species (ROS), many plants have developed some unique systems to alleviate the ROS production and mitigate its deleterious effect. One of the key pathways that gets activated in plants is ascorbate glutathione (AsA-GSH) pathway.

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Exogenously supplied BR and JA help KS101 and KBS3 genotypes of Brassica rapa to alleviate drought stress by modifying osmolyte concentration, levels of antioxidant enzymes and photosynthetic system. Oilseed plants are susceptible to drought stress and a significant loss in yield has been reported during recent decades. Thus, it is imperative to understand the various underlying drought response mechanisms in Brassica oilseed plants to formulate the sustainable strategies to protect the crop yield under water-limiting conditions.

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Unlabelled: Maize, a C sub-tropical crop, possesses higher temperature optima as compared to the C plants. Low temperature (LT) stress confines the growth and productivity of maize. In this context, two maize genotypes, LT tolerant Gurez local and LT susceptible Gujarat-Maize-6 (G-M-6) were analysed in present study for various osmolytes and gene expression of antioxidant enzymes including Ascorbate-glutathione (AsA-GSH) besides trehalose biosynthetic pathways.

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Maize is a low-temperature (LT)-sensitive plant and its physiological responses towards LT of temperate regions developed is an adaptive trait. To further our understanding about the response of maize to LT at the physiological and photosynthesis level, we conducted Infrared Gas Analysis (IRGA using LICOR6400-XT in 45-day-old grown two maize genotypes, one from temperate region (Gurez-Kashmir Himalayas), viz., Gurez local (Gz local), and another from tropics (Gujarat), viz.

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Environmental pollution by alkaline salts, such as NaCO, is a permanent problem in agriculture. Here, we examined the putative role of jasmonic acid (JA) in improving NaCO-stress tolerance in maize seedlings. Pretreatment of maize seedlings with JA was found to significantly mitigate the toxic effects of excessive NaCO on photosynthesis- and plant growth-related parameters.

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Topoisomerases are unique enzymes having an ability to remove or add DNA supercoils and untangle the snarled DNA. They can cut, shuffle, and religate DNA strands and remove the torsional stress during DNA replication, transcription or recombination events. In the present study, we over-expressed topoisomerase II (TopoII) in tobacco (Nicotiana tabaccum) and examined its role in growth and development as well as salt (NaCl) stress tolerance.

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Salinity stress affected crop production of more than 20% of irrigated land globally. In the present study the effect of different concentrations of NaCl (0, 100, and 200 mM) on growth, physio-biochemical attributes, antioxidant enzymes, oil content, etc. in Brassica juncea and the protective role of Trichoderma harzianum (TH) was investigated.

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Article Synopsis
  • * Immuno-localization studies show that Topo I is concentrated in the nucleolus and correlates with ribosomal RNA transcription activity, indicating its importance in gene expression.
  • * Research indicates that while Topo I is vital for DNA processes, Topo II is essential during chromosome condensation, and experiments with transgenic plants suggest Topo I is crucial for proper plant growth and development.
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Article Synopsis
  • - Molecular breeding is essential for enhancing crop production, as traditional breeding methods have not effectively increased food supply, while advancements like next-generation sequencing have opened new avenues.
  • - Exome sequencing is highlighted as a vital tool for understanding plant evolution, disease interactions, and crop improvement by analyzing allelic variations and their phenotypic effects.
  • - The review discusses advancements in sequencing technologies and their applications in boosting yields in crops like wheat, rice, and cotton, along with exploring biodiversity and enhancing our understanding of plant-pathogen dynamics, while emphasizing the need for integrating various methodologies for comprehensive agricultural advancements.
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Growth, lipid peroxidation, different antioxidative enzymes and metal accumulation were studied in Lemna polyrrhiza treated with different concentrations (1-40 ppm) of CdSO4. The growth of the plant was slightly enhanced with 1 ppm, while higher concentrations retarted growth and multiplication of fronds, the effect being concentration and dose dependant. Increase in malondialdehyde content was insignificant after the first week but a prolonged exposure led to significant (p < 0.

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