Aim: The aim of this study was to evaluate the effects of various combinations of tropical grass-legume species in rations on the biohydrogenation (BH) activity of unsaturated fatty acids (FAs), C18:0 composition, and fermentation profile in an rumen system.
Materials And Methods: Samples of the following five fodder plants were used: One species of grass () and four species of tree legumes (, , , and ). The following eight experimental diets were evaluated: 50% + 50% (LL I); 50% + 50% (GS I); 50% + 50% (CC I); 50% + 50% (IZ I); 75% + 25% (LL II); 75% + 25% (GS II); 75% + 25% (CC II); and 75% + 25% (IZ II).