Unveiling the Bioactive Potential of Bacterial Isolates from Extreme Environments of Pakistan by In Vitro and In Silico Approaches.

The soil hosts a wide array of bacterial species capable of producing diverse bioactive compounds. This research aimed to screen bacterial isolates for their bioactive potential from extreme environments in Pakistan. Out of the 69 isolates examined, only 7 exhibited antagonistic activity against Bacillus sp.

Impact of internal identity asymmetry on employee's behaviors and feelings: A mediating role of psychological distress.

Individuals may experience internal identity asymmetry when they feel misidentified and believe their colleagues do not recognize their work-related identities. This research examines the impact of internal identity asymmetry on their behavior and emotional outcomes at the workplace in Pakistan. Data was collected through a survey and received responses from 393 participants at different levels of management in various sectors of Pakistan.

Acyclovir-Induced Nephrotoxicity in Adults with Viral Encephalitis.

Objective: To determine the frequency of parenteral Acyclovir-induced Acute Kidney Injury (AKI) in patients with viral encephalitis.

Study Design: Descriptive study. Place and Duration of the Study: Department of Neurology, Liaquat National Hospital, Karachi, from January to December 2021.

Fate and potential risks of microplastic fibers and fragments in water and wastewater treatment processes.

Water and wastewater treatment plants (WWTPs) receive various types of microplastics (MPs), with fibers and fragments being dominant shapes. Here we investigated the removal behavior and transformation of MPs (polypropylene and polyethylene terephthalate fibers and fragments) in simulated water and wastewater treatment units, including activated sludge process, coagulation, sand filtration, and advanced oxidation/disinfection. Sand filtration demonstrated the highest average efficiency in removing MPs (98 %), followed by activated sludge process (61 %) and coagulation (55 %), which was associated with their physicochemical properties (shape, size, density, surface functional groups, etc).

Chromate Removal by Enterobacter cloacae Strain UT25 from Tannery Effluent and Its Potential Role in Cr (VI) Remediation.

An indigenous chromate-resistant bacterial strain isolated from tannery effluent was identified based on morphological, biochemical, and 16S rRNA gene sequencing, as Enterobacter cloacae UT25. It was found to resist heavy metal ions such as Cr (VI), Pb (II), Cu (II), Co (II), Ni (II), Hg (II), and Zn (II) and antibiotics. The strain was able to remove 89 and 86% chromate, after 24 h of incubation in a Luria-Bertani (LB) medium at an initial Cr (VI) concentration of 1000 and 1500 µg/ml, respectively.

Co-Culture of Halotolerant Bacteria to Produce Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Using Sewage Wastewater Substrate.

The focus of the current study was the use of sewage wastewater to obtain PHA from a co-culture to produce a sustainable polymer. Two halotolerant bacteria, 14SM (MZ801771) and WK31 (MT453992), were grown in a consortium to produce PHA. Sewage wastewater (SWW) was used to produce PHA, and glucose was used as a reference substrate to compare the growth and PHA production parameters.

Evaluating the Role of Wastewaters as Reservoirs of Antibiotic-Resistant ESKAPEE Bacteria Using Phenotypic and Molecular Methods.

Introduction: Wastewaters carrying thousands of human specimens from the community and representing the diversity of antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs) directly from the community mirror the extent of AR spread in the community and environment. This study aimed to investigate the occurrence and distribution of antibiotic-resistant ESKAPEE bacteria in the community versus clinical settings through monitoring nonclinical and clinical wastewaters.

Methodology: Seven wastewater samples were collected from different environmental sources.

Seasonal variation and spatial distribution of microplastics in tertiary wastewater treatment plant in South Korea.

Wastewater treatment plant (WWTP) is proposed to be a point source of microplastics (MPs) that enter aquatic environments. Here, we quantified and characterized MPs down to 20 µm at a tertiary WWTP in Korea over a 1-year period. All wastewater contained MPs during the monitoring period, with concentrations ranging from 114 ± 17-216 ± 65 particles/L for influent and from 0.

Biodeterioration of Microplastics: A Promising Step towards Plastics Waste Management.

Polyethylene and Polyester materials are resistant to degradation and a significant source of microplastics pollution, which is an emerging concern. In the present study, the potential of a dumped site bacterial community was evaluated. After primary screening, it was observed that 68.

COVID-19 severity: Studying the clinical and demographic risk factors for adverse outcomes.

Background: The primary goal of the presented cross-sectional observational study was to determine the clinical and demographic risk factors for adverse coronavirus disease 2019 (COVID-19) outcomes in the Pakistani population.

Methods: We examined the individuals (n = 6331) that consulted two private diagnostic centers in Lahore, Pakistan, for COVID-19 testing between May 1, 2020, and November 30, 2020. The attending nurse collected clinical and demographic information.

Bursting the Virulence Traits of MDR Strain of Using Sodium Alginate-based Microspheres Containing Nystatin-loaded MgO/CuO Nanocomposites.

Introduction: is a major opportunistic pathogen that causes a wide range of human infections. Currently available therapeutic agents are limited for treating these fungal infections due to multidrug resistance as well as their nonbiodegradability, poor biocompatibility and toxicity. In order to battle these limitations, we have synthesized a polymeric system as microcarriers to deliver the antifungal drug.

Novel N-p-carboxy benzyl chitosan/poly (vinyl alcohol/functionalized zeolite mixed matrix membranes for DMFC applications.

The novel N-p-carboxy benzyl chitosan (CBC)/ poly (vinyl alcohol) (PVA) based mixed matrix membranes (MMMs) filled with surface-modified zeolite have been prepared using the dissolution casting technique. The applicability of prepared MMMs for direct methanol fuel cell (DMFC) was investigated in terms of water uptake, methanol permeation, and proton conductivity by changing filler content (10-50 wt. %).

Phytochemical screening and antibacterial potential of Artemisia absinthium L., Swertia chirayita and Sphaeranthus indicus.

Utilization of herbs for medicinal purpose started in the early history of mankind several thousand years ago. In this study, some plants that are used for lowering cholesterol level in local areas of Pakistan, such as Artemisia absinthium L., Swertia chirayita and Sphaeranthus indicus were screened for their phytochemical and antibacterial properties.

Antilisterial Effect of and in .

The present study was proposed to investigate the toxicological and prophylactic potential of ethanolic extracts of and and their mixture in albino mice. For toxicity study, three different doses of plant extracts were orally administrated to three groups of mice for 14 successive days. Blood biochemistry and histological examinations of liver and kidney revealed that these extracts had no harmful effects up to 1000 mg/kg.

Production of EPS under Cr(VI) challenge in two indigenous bacteria isolated from a tannery effluent.

Indigenous Cr(VI) reducing bacterial strains Pseudomonas aeruginosa Rb-1 and Ochrobactrum intermedium Rb-2 were evaluated for EPS production under Cr(VI) challenged and free conditions. Strain Rb-2 was more efficient in total EPS production (13.63 mg g(-1)) than Rb-1 (4.

Impact of environmental stress on biochemical parameters of bacteria reducing chromium.

Chromium pollution is produced in connection with industrial processes like in tanneries. It has been suggested that bioremediation could be a good option for clean up. The stress effect of variable chromate levels, pHs and growth temperatures on biochemical parameters of two Cr(VI) reducing bacterial strains Pseudomonas aeruginosa Rb-1 and Ochrobactrum intermedium Rb-2 was investigated.

Hexavalent chromium reduction by bacteria from tannery effluent.

Chromium is generated from several industrial processes. It occurs in different oxidation states, but Cr(III) and Cr(VI) are the most common ones. Cr(VI) is a toxic, soluble environmental contaminant.