Publications by authors named "Richard W Doty"
Article Synopsis
- Recent advances in single-cell RNA sequencing (scRNA-seq) and CRISPR technology allow researchers to explore how genetic changes affect gene expression in a high-throughput manner.
- A key challenge in these experiments is managing "ambient gRNAs," which are extraneous genetic markers from other cells that can skew results if not filtered out properly.
- The study introduces a tool called CLEANSER, which effectively distinguishes and removes ambient gRNA noise, improving the accuracy of gRNA-cell assignments and enhancing the quality of differential gene expression analyses.
Diabetic retinopathy is a potentially blinding eye disease that threatens the vision of one-ninth of patients with diabetes. Progression of the disease has long been attributed to an initial dropout of pericytes that enwrap the retinal microvasculature. Revealed through retinal vascular digests, a subsequent increase in basement membrane bridges was also observed.
View Article and Find Full Text PDFAlterations in vascular networks, including angiogenesis and capillary regression, play key roles in disease, wound healing, and development. The spatial structures of blood vessels can be captured through imaging, but effective characterization of network architecture requires both metrics for quantification and software to carry out the analysis in a high-throughput and unbiased fashion. We present Rapid Editable Analysis of Vessel Elements Routine (REAVER), an open-source tool that researchers can use to analyze high-resolution 2D fluorescent images of blood vessel networks, and assess its performance compared to alternative image analysis programs.
View Article and Find Full Text PDFMyh11 Lineage Corneal Endothelial Cells and ASCs Populate Corneal Endothelium.
Invest Ophthalmol Vis Sci
December 2019
Purpose: To establish Myh11 as a marker of a subset of corneal endothelial cells (CECs), and to demonstrate the feasibility of restoring the corneal endothelium with Myh11-lineage (Myh11-Lin[+]) adipose-derived stromal cells (ASCs).
Methods: Intraperitoneal administration of tamoxifen and (Z)-4-hydroxytamoxifen eyedrops were used to trace the lineage of Myh11-expressing cells with the Myh11-Cre-ERT2-flox-tdTomato mouse model. Immunostaining and Western blot characterized marker expression and spatial distribution of Myh11-Lin(+) cells in the cornea, and administration of 5-ethynyl-2'-deoxyuridine labeled proliferating cells.