Dynamin function is important for chemokine receptor-induced cell migration.

The HIV viral entry co-receptors CCR5 and CXCR4 function physiologically as typical chemokine receptors. Activation leads to cytosolic signal transduction that results in a variety of cellular responses such as cytoskeletal rearrangement and chemotaxis (CTX). Our aim was to investigate the signalling pathways involved in CC and CXC receptor-mediated cell migration.

Differential regulation of chemotaxis: role of Gβγ in chemokine receptor-induced cell migration.

CC and CXC chemokine receptor signalling networks are regulated in different ways. Here we show that intracellular calcium release and cell migration occur independent of Gβγ activation in response to CCL3, whereas CXCL11 induced migration of activated T-lymphocytes depends on Gβγ activation. Treatment of a range of cell types with gallein, a pharmacological inhibitor of Gβγ signalling, did not result in a reduction in CCL3 induced cellular migration, but resulted in enhanced calcium mobilisation following chemokine stimulation.