E-selectin and ICAM-1 are incorporated into detergent-insoluble membrane domains following clustering in endothelial cells.

Here we present data supporting the role of lipid rafts in endothelial cells during leukocyte adhesion. Following adhesion of THP-1 cells or antibody-mediated clustering, both E-selectin and intercellular adhesion molecule-1 (ICAM-1) partitioned into the detergent-insoluble portion of the endothelial cellular lysate. Sucrose gradient centrifugation revealed the partitioning of clustered E-selectin and ICAM-1 with the low-density fraction where they co-fractionated with src family kinases, markers of lipid rafts.

The Src-cortactin pathway is required for clustering of E-selectin and ICAM-1 in endothelial cells.

Adhesion molecules such as E-selectin and intercellular adhesion molecule-1 (ICAM-1) expressed on endothelial cells (ECs) at sites of inflammation play an important role in the recruitment of leukocytes from the bloodstream into extravascular tissue. However, little is known about the signaling pathways that are initiated in ECs following adhesion molecule engagement. Here, we report that an 85-kDa protein becomes tyrosine phosphorylated in human ECs following leukocyte adhesion or upon antibody-induced clustering of E-selectin or ICAM-1.

The Faculty Athletic Training Program: A Model.

In brief Most high schools lack funds to hire a full-time trainer. This article describes a pilot program that trained 43 employed teachers to become certified athletic trainers. After completing the course, 40 of the 43 are currently working either as formally recognized athletic trainers or are bringing more understanding of injury prevention, recognition, and care to their teaching and coaching roles.

Sportsmedicine Care in Chicago-Area High Schools.

Since few high schools have trainers on staff, Ms Porter studied the approach to injury management in 191 schools.