The Nemourinae (Insecta, Nemouridae) of the eastern Nearctic.

Morphology-based concepts of the 26 eastern Nearctic species of the subfamily Nemourinae (Insecta, Nemouridae) Billberg, 1820 are reviewed. Nemourinae is represented in this region by Nemoura Latrielle, 1796, Ostrocerca Ricker, 1952, Paranemoura Needham & Claassen, 1925, Podmosta Ricker, 1952, Prostoia Ricker, 1952, Shipsa Ricker, 1952, Soyedina Ricker, 1952, and Zapada Ricker, 1952. Wing venation and patterns of mottling are depicted with standard light microscopy.

Dracoleuctra, a new wingless stonefly genus from southern Oregon, U.S.A. (Plecoptera: Leuctridae).

Dracoleuctra siskiyou Lee Baumann gen. nov. sp.

Soyedina Ricker, 1952 (Plecoptera: Nemouridae) in the eastern Nearctic: review of species concepts, proposed morphology-based species groups, and description of a new species from North Carolina.

The eastern Nearctic species of the genus Soyedina Ricker, 1952 (Plecoptera: Nemouridae) are reviewed. Two morphology-based species groups are proposed based on epiproct characteristics. Soyedina sheldoni sp.

Alloperla clarki sp. nov. (Plecoptera: Chloroperlidae), a new species from the eastern Nearctic with discussion of a new species group.

Alloperla Banks, 1906 (Plecoptera: Chloroperlidae) is a speciose genus of spring- and summer-emergent stoneflies known from the Oriental, eastern Palearctic, and Nearctic realms. Over 50 species are currently recognized. Alloperla clarki sp.

Atlas of Ohio Aquatic Insects: Volume II, Plecoptera.

Background: We provide volume II of a distributional atlas of aquatic insects for the eastern USA state of Ohio. This treatment of stoneflies (Plecoptera) is companion to Armitage et al. (2011) on caddisflies (Trichoptera).

A review of the Nearctic genus Prostoia (Ricker) (Plecoptera, Nemouridae), with the description of a new species and a surprising range extension for P. hallasi Kondratieff & Kirchner.

The Nearctic genus Prostoia (Plecoptera: Nemouridae) is reviewed. Prostoia ozarkensis sp. n.

Time-dependent changes in serum biomarker levels after blast traumatic brain injury.

Neuronal and glial proteins detected in the peripheral circulating blood after injury can reflect the extent of the damage caused by blast traumatic brain injury (bTBI). The temporal pattern of their serum levels can further predict the severity and outcome of the injury. As part of characterizing a large-animal model of bTBI, we determined the changes in the serum levels of S100B, neuron-specific enolase (NSE), myelin basic protein (MBP), and neurofilament heavy chain (NF-H).

Selection of cholera toxin specific IgNAR single-domain antibodies from a naïve shark library.

Shark immunoglobulin new antigen receptor (IgNAR, also referred to as NAR) variable domains (Vs) are single-domain antibody (sdAb) fragments containing only two hypervariable loop structures forming 3D topologies for a wide range of antigen recognition and binding. Their small size ( approximately 12kDa) and high solubility, thermostability and binding specificity make IgNARs an exceptional alternative source of engineered antibodies for sensor applications. Here, two new shark NAR V display libraries containing >10(7) unique clones from non-immunized (naïve) adult spiny dogfish (Squalus acanthias) and smooth dogfish (Mustelus canis) sharks were constructed.

Portal fusion protein constraints on function in DNA packaging of bacteriophage T4.

Architecturally conserved viral portal dodecamers are central to capsid assembly and DNA packaging. To examine bacteriophage T4 portal functions, we constructed, expressed and assembled portal gene 20 fusion proteins. C-terminally fused (gp20-GFP, gp20-HOC) and N-terminally fused (GFP-gp20 and HOC-gp20) portal fusion proteins assembled in vivo into active phage.

Selective anterior fusion of thoracolumbar/lumbar curves in adolescents: when can the associated thoracic curve be left unfused?

Study Design: A retrospective multicenter study was conducted to investigate patients with a major thoracolumbar/lumbar adolescent idiopathic scoliosis and an associated minor thoracic curve treated with an anterior instrumentation and fusion of the lower curve.

Objective: To establish criteria for determining when such curves can be successfully treated by an anterior only procedure of the lower curve with acceptable spinal balance and residual thoracic curve.

Summary Of Background Data: Anterior spinal instrumentation techniques have been proved effective for the management of isolated thoracolumbar/lumbar scoliosis with small compensatory thoracic curves.

Isolation and characterization of T4 bacteriophage gp17 terminase, a large subunit multimer with enhanced ATPase activity.

Phage T4 terminase is a two-subunit enzyme that binds to the prohead portal protein and cuts and packages a headful of concatameric DNA. To characterize the T4 terminase large subunit, gp17 (70 kDa), gene 17 was cloned and expressed as a chitin-binding fusion protein. Following cleavage and release of gp17 from chitin, two additional column steps completed purification.

A bipartite bacteriophage T4 SOC and HOC randomized peptide display library: detection and analysis of phage T4 terminase (gp17) and late sigma factor (gp55) interaction.

HOC and SOC are dispensable T4 capsid proteins that can be used for phage display of multiple copies of peptides and proteins. A bipartite phage T4 peptide library was created by displaying on tetra-alanine linker peptides five randomized amino acids from the carboxyl-terminus of SOC and five randomized amino acids from the amino terminus of HOC. The bipartite library was biopanned against the phage T4 terminase large subunit gp17 to identify T4 gene products that may interact with the terminase.