Structure of the Bacterial Community in Different Stages of Early Childhood Caries.

Purpose: To characterise in vivo the structure of bacterial communities in decayed and sound primary teeth.

Materials And Methods: Samples of biofilms were collected from three groups of patients with complete and exclusively primary dentition (n = 45): G1: sound teeth (n = 15); G2: enamel lesion (n = 15); G3: dentin lesion (n = 15). DNA was extracted (CTAB 2%) from the biofilm, the partial 16S rRNA gene was amplified with Bacteria Universal Primers (BA338fGC - UN518r) and subjected to DGGE (denaturing gradient gel electrophoresis).

Bonding of fractured permanent central incisor crown following radiographic localization of the tooth fragment in the lower lip: a case report.

This study describes the case of an 8-year-old boy who fractured his left maxillary central incisor, exposing the pulp. The trauma also resulted in a cut on his mentum. Radiographic examination revealed the fractured tooth fragment embedded in the lower lip.

Shear bond strength of different adhesive systems to primary dentin and enamel.

The aims of this study were to evaluate the shear bond strength (SBS) off our adhesive systenms applied to primary dentin and enamel and verify, after SBS testing, the failure mode of the adhesive interface. Sixty extracted sound primary molars were selected and crowns were sectioned in a mesial-distal direction. Specimens were randomly assigned into two groups (adhesion to enamel and adhesion to dentin) and then subdivided into four subgroups according to the adhesive system (n=15).