Publications by authors named "Ricardo Silva"

Adeno-associated viruses (AAV) are becoming increasingly popular as a powerful tool for gene delivery therapy applications. Although processes to produce AAV are established, future demand for this type of viral vector requires further development of manufacturing processes to make them more robust, scalable, and flexible to accommodate the rise of engineered capsids. This study focuses on designing and evaluating a two-step chromatography process for capturing and polishing AAV8 using monolith chromatography media.

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Oyster mushroom (Pleurotus ostreatus) is the second most produced mushroom globally, but increased production is linked to new diseases. This study reports the first occurrence of Penicillium brevicompactum on P. ostreatus mushrooms.

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This study evaluated the bond strength (BS) of conventional, anatomized, adjustable, and CAD/CAM fiberglass post systems. Forty maxillary canine roots were weakened, endodontically treated, and divided into four groups (n=10). A slice from each of the three sections (cervical, middle, apical) of the root canal was subjected to the push-out test and the failure pattern was subsequently analyzed.

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  • Paropsisterna bimaculata is a harmful pest for eucalyptus trees, leading to significant defoliation and even tree death in Tasmanian plantations, impacting wood production.
  • The study focused on creating an ecoclimatic index model using CLIMEX software to predict where this pest might spread globally, identifying high-risk areas for eucalyptus crops.
  • Results indicated that regions with temperate climates across Asia, Africa, Europe, Oceania, and America are especially vulnerable, with a notable risk present in Brazil's southern and southeastern areas, guiding prevention strategies.
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The Hepatitis B core antigen (HBcAg) has been used as a carrier of several heterologous protein fragments based on its capacity to form virus-like particles (VLPs) and to activate innate and adaptive immune responses. In the present work, two chimeric proteins were designed as potential pancorona vaccine candidates, comprising the N- or C- terminal domain of SARS-CoV-2 nucleocapsid (N) protein fused to HBcAg. The recombinant proteins, obtained in E.

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  • - Untargeted metabolomics, often reliant on data-dependent acquisition (DDA), has limitations in fragmenting all detected ions and specificity, prompting the development of a dynamic procedure to enhance detection of biological signals.
  • - The study analyzed three species of actinomycetes using liquid chromatography and mass spectrometry, employing MZmine for preprocessing and a custom tool, RegFilter, for data processing.
  • - RegFilter improved ion detection by covering the whole chromatographic run and allowed for the annotation of biologically relevant candidates, thus serving as a valuable complement to DDA and enabling the creation of customized spectral libraries.
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Introduction: The diagnostic conversion of major depressive disorder (MDD) to bipolar disorder (BD) is a topic that is currently the subject of several studies. However, there are few studies that clarify the interaction between conversion, substance use and biomarkers.

Objectives: The aim of this study was to investigate serum biomarker levels and lifetime substance use as predictors for diagnostic conversion from major depressive disorder to bipolar disorder in an outpatient sample of adults.

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Patients with peripheral artery disease and generalized atherosclerosis are at high risk of cardiovascular and limb complications, affecting both quality of life and longevity. Lower limb atherosclerotic disease is associated with high cardiovascular morbidity and mortality and adequate management is founded on treatments involving patient-dependent factors, such as lifestyle changes, and physician-dependent factors, such as clinical treatment, endovascular treatment, or conventional surgery. Medical management of peripheral artery disease is multifaceted, and its most important elements are reduction of cholesterol level, antithrombotic therapy, control of arterial blood pressure, control of diabetes, and smoking cessation.

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  • This study aimed to analyze the relationships between emotional regulation difficulties and eating disorders (EDs) symptoms in college students, identifying them as a high-risk group.
  • Researchers used a network analysis with 294 college students to understand how various psychological factors (like self-compassion and mindfulness) interact concerning eating psychopathology.
  • Findings revealed that disordered eating symptoms were closely linked to interoceptive awareness, with self-compassion being critical for emotional regulation, suggesting that improving these areas could help prevent EDs in at-risk students.
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Postmortem interval (PMI) estimates the time since death. Teeth are perennial elements capable of remaining intact in taphonomic environmental circumstances. The objective was to evaluate the feasibility of estimating the minimum postmortem interval (PMI) through histological analyses of dental tissues exposed to burial and drowning conditions, simulating common scenarios in forensic practice.

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  • Climate change is projected to affect the habitat suitability of invasive species, like the mango stone weevil (Sternochetus mangiferae), hindering early detection and preventive strategies due to limited existing data.
  • The MaxEnt model shows that thermal conditions significantly influence S. mangiferae's invasion risk, with suitable habitats found on every continent except Antarctica; however, these areas are projected to decrease in the coming decades.
  • This research lays the groundwork for assessing the global risk posed by the mango stone weevil and helps identify regions more vulnerable to climate change impacts, aiding in the forecasting of its distribution.
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  • Thyrinteina arnobia is an invasive pest originally from America known to affect Eucalyptus plantations, but there's limited information on its population diversity and ecology in Brazil.
  • A study showed that T. arnobia has low genetic diversity across Brazil, with distinct mitochondrial haplogroups in different regions, indicating pest outbreaks are localized and not due to long-distance spread.
  • Climate factors like isothermality and annual rainfall are key to T. arnobia's distribution, suggesting it could pose a threat to Eucalyptus forests both in Brazil and in other tropical and subtropical areas around the world.
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This work aimed to synthesize a new pyrimidine with potential application against antimicrobial resistance. was synthesized through condensation reaction between and . The antimicrobial evaluation was carried out using the microdilution method in Mueller-Hinton Agar and predictions using different software.

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Chagas disease, a neglected global health concern primarily transmitted through the bite and feces of kissing bugs, has garnered increasing attention due to recent outbreaks in northern Brazil, highlighting the role of oral transmission facilitated by the kissing bugs species Rhodnius robustus and Rhodnius pictipes. These vectors are associated with palm trees with large crowns, such as the maripa palm (Attalea maripa) and moriche palm (Mauritia flexuosa). In this study, we employ maximum entropy (MaxEnt) ecological niche models to analyze the spatial distribution of these vectors and palm species, predicting current and future climate suitability.

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Objective: to describe factors of influence of telenursing on naturalistic decision making about self-care of people with heart failure during COVID-19.

Method: this is a descriptive study with a qualitative approach, with 16 participants interviewed after hospitalization for decompensated heart failure. The data was collected using audio and video technology and submitted to content analysis, guided by the Situation-Specific Theory of Heart Failure Self-Care.

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Reverse left ventricular (LV) remodeling after aortic valve replacement (AVR), in patients with aortic stenosis, is well-documented as an important prognostic factor. With this systematic review and meta-analysis, we aimed to characterize the response of the unloaded LV after AVR. We searched on MEDLINE/PubMed and Web of Science for studies reporting echocardiographic findings before and at least 1 month after AVR for the treatment of aortic stenosis.

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Corn () is the most widely planted crop in the world. (Hemiptera: Cicadellidae) is currently a primary corn pest. The starting point for the development of pest control decision-making systems is the determination of a conventional sampling plan.

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Objectives: The emergence of SARS-CoV-2 has triggered a global pandemic with profound implications for public health. Rapid changes in the pandemic landscape and limitations in diagnostics led to the introduction of numerous diagnostic devices with variable performance. In this study, we evaluated three commercial serological assays in Brazil for detecting anti-SARS-CoV-2 antibodies.

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Coastal regions are sectors where human activities impact the marine ecosystem, and if necessary control measures are not taken, they can generate negative consequences for health and ecosystem services. Within the framework of the Pampa Azul initiative and under the One Health paradigm, the interconnection between the terrestrial and marine environments of the San Jorge Gulf and the adjacent north coast has been studied. In November, 2022, a campaign was carried out aboard the R/V "Mar Argentino" at thirty-four stations near the coast.

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A chimeric protein, formed by two fragments of the conserved nucleocapsid (N) and S2 proteins from SARS-CoV-2, was obtained as a recombinant construct in . The N fragment belongs to the C-terminal domain whereas the S2 fragment spans the fibre structure in the post-fusion conformation of the spike protein. The resultant protein, named S2NDH, was able to form spherical particles of 10 nm, which forms aggregates upon mixture with the CpG ODN-39M.

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This manuscript presents a new report on the in vitro antimicrobial photo-inactivation of foodborne microorganisms (Salmonella spp. and Listeria monocytogenes) using tetra-cationic porphyrins. Isomeric tetra-cationic porphyrins (3MeTPyP, 4MeTPyP, 3PtTPyP, and 4PtTPyP) were tested, and antimicrobial activity assays were performed at specific photosensitizer concentrations under dark and white-light LED irradiation conditions.

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Background: Tools for assessing a country's capacity in the face of public health emergencies must be reviewed, as they were not predictive of the COVID-19 pandemic. Social cohesion and risk communication, which are related to trust in government and trust in others, may have influenced adherence to government measures and mortality rates due to COVID-19.

Objective: To analyse the association between indicators of social cohesion and risk communication and COVID-19 outcomes in 213 countries.

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Effective radical cure of Plasmodium vivax malaria is essential for malaria elimination in Brazil. P. vivax radical cure requires administration of a schizonticide, such as chloroquine, plus an 8-aminoquinoline.

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Synthetic hydrogels provide controllable 3D environments, which can be used to study fundamental biological phenomena. The growing body of evidence that cell behavior depends upon hydrogel stress relaxation creates a high demand for hydrogels with tissue-like viscoelastic properties. Here, a unique platform of synthetic polyethylene glycol (PEG) hydrogels in which star-shaped PEG molecules are conjugated with alendronate and/or RGD peptides, attaining modifiable degradability as well as flexible cell adhesion, is created.

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