Publications by authors named "Ricardo Domingo-Breton"
Oxford Nanopore Technology (ONT) allows for the rapid profiling of aquaculture microbiomes. However, not all the experimental and downstream methodological possibilities have been benchmarked. Here, we aimed to offer novel insights into the use of different library preparation methods (standard-RAP and native barcoding-LIG), primers (V3-V4, V1-V3, and V1-V9), and basecalling models (fast-FAST, high-HAC, and super-accuracy-SUP) implemented in ONT to elucidate the microbiota associated with the aquatic environment and farmed fish, including faeces, skin, and intestinal mucus.
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- A confinement stress test was conducted to analyze the stress response in gilthead sea bream compared to European sea bass, focusing on behavioral changes using three-axis accelerometers.
- The study involved rearing gilthead sea bream at different stocking densities and monitoring various health metrics, showing that fish in high-density (HD) conditions had lower feed intake and growth rates, along with detectable muscle changes and minor injuries.
- After undergoing stress tests, fish in high-density settings demonstrated quicker recovery in activity and respiration, suggesting they became accustomed to the high stocking environment over time.