Publications by authors named "Ricardo A Reis"

The objective of this study was to evaluate the effect of 3-nitrooxypropanol (3-NOP) in combination with different feed additives on growth performance, carcass traits, meat quality, enteric methane (CH) emissions, nutrient intake and digestibility, and blood parameters in feedlot beef cattle. In experiment (Exp.) 1, one hundred sixty-eight Nellore bulls (initial bodyweight (BW) 410 ± 8 kg) were allocated to 24 pens in a completely randomized block design.

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Article Synopsis
  • * Key factors affecting nitrogen excretion included dry matter intake, nitrogen intake, dietary composition, and animal growth metrics; models were evaluated using statistical measures like root mean square error (RMSE) and concordance correlation coefficient (CCC).
  • * While fecal N excretion models showed better accuracy than those for urinary excretion, different predictors led to varying performances, with nitrogen intake generally performing better for urine, indicating the complexity of predicting N excretion based on diet and management.
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Grasslands cover approximately 24% of the Earth's surface and are the main feed source for cattle and other ruminants. Sustainable and efficient grazing systems require regular monitoring of the quantity and nutritive value of pastures. This study demonstrates the potential of estimating pasture leaf forage mass (FM), crude protein (CP) and fiber content of tropical pastures using Sentinel-2 satellite images and machine learning algorithms.

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This study aimed to evaluate the effects of supplementation with non-protein nitrogen (NPN) or ruminal undegradable protein (RUP) on intake, digestibility, and amino acid (AA) use efficiency of Nellore cattle grazing during the dry season. Eight Nellore steers (12 ± 2 months old) were used in quadruplicate Latin squares (2 × 2). The animals were placed on cv.

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Introduction: High quality corn silage depends on factors such as corn type, stage of crop development at harvest time, fermentation time, in addition to use or not of inoculants. This study aimed to investigate the impact of maturity stage, bacterial inoculation, and storage time on fermentation, aerobic stability, and nutritional characteristics of flint corn silage and their implications for corn silage management.

Methods: A flint corn hybrid was harvested very early, early, and medium (at 250, 300 and 350 g dry matter (DM)/kg as fed, respectively) and ensiled in mini-silos without (control) or with CNCM I-4323 at 1 × 10 cfu/g for 120, 240 and 360 d to investigate how these factors interact with each other.

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Our objective was to evaluate the effects of feeding 3-nitrooxypropanol (3-NOP; Bovaer, DSM Nutritional Products) at two levels on methane emissions, nitrogen balance, and performance by feedlot cattle. In experiment 1, a total of 138 Nellore bulls (initial body weight, 360 ± 37.3 kg) were housed in pens (27 pens with either 4 or 5 bulls per pen) and fed a high-concentrate diet for 96 d, containing 1) no addition of 3-NOP (control), 2) inclusion of 3-NOP at 100 mg/kg dry matter (DM), and 3) inclusion of 3-NOP at 150 mg/kg DM.

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The objective of this study was to evaluate the effect of the addition of a phytogenic compound blend (PHA) containing hydrolyzable tannins, carvacrol, and cinnamaldehyde oil to mineral salt or energy supplementation on the rumen microbiota and nitrogen metabolism of grazing Nellore cattle. Eight castrated Nellore steers were distributed in a double-Latin-square 4 × 4 design, with a 2 × 2 factorial arrangement (two types of supplements with or without the addition of the PHA), as follows: energy supplement without the PHA addition (EW); energy supplement with the PHA addition (EPHA); mineral supplement without the addition of the PHA (MW); mineral supplement with the PHA addition (MPHA). Steers that received supplements with the PHA have a lower ruminal proportion of valerate (with the PHA, 1.

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Grazing management and N-fertilizer have been reported to improve tropical forage productivity and quality, however, their effect on methane emission of grazing animals remains uncertain. Therefore, this study aimed to assess the effects of increasing application rates of nitrogen (N) fertilization of Marandu palisadegrass under continuous stocking on intake, digestibility, nitrogen balance, and enteric methane emissions of Nellore growing bulls. We hypothesized that changes in the forage nutritive value caused by N fertilization of pastures combined with adequate grazing management (e.

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On-farm methane (CH) emissions need to be estimated accurately so that the mitigation effect of recommended practices can be accounted for. In the present study prediction equations for enteric CH have been developed in lieu of expensive animal measurement approaches. Our objectives were to: (1) compile a dataset from individual beef cattle data for the Latin America and Caribbean (LAC) region; (2) determine main predictors of CH emission variables; (3) develop and cross-validate prediction models according to dietary forage content (DFC); and (4) compare the predictive ability of these newly-developed models with extant equations reported in literature, including those currently used for CH inventories in LAC countries.

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Condensed tannins are a potentially important treatment option to mitigate NO (nitrous oxide) and affect carbon dioxide (CO) and methane (CH) emissions; however, their effect has been poorly assessed. Here, we quantified the emissions of NO, CH, and CO, soil N mineralization, and nitrification with increasing doses of condensed tannins added to the urine of cattle raised on pasture. The experiment consisted of incubation with doses of 0%, 0.

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The wet-dry transition period brings unique challenges to cattle producers in the tropics as the forage quality starts to decrease and animal performance is negatively affected unless supplementation strategies are applied. Two experiments were conducted concomitantly to evaluate the performance and metabolic parameters of cattle supplemented with two different energy sources under a C4 tropical grass continuous grazing system in the wet-dry transition period. In experiment (exp) 1, the liveweight (LW) gain of 42 growing Nellore bulls allocated to 12 paddocks of cv.

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This study evaluated intake, apparent digestibility, ruminal parameters, nitrogen balance, and microbial protein synthesis in grazing beef cattle fed a mineral mix or combination of two coproducts (cottonseed meal and dried distiller's grains (DDG)) during the wet season. Urochloa brizantha cv. Marandu pastures were managed under continuous stocking to maintain a fixed grazing height of 25 cm using put-and-take methodology.

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The aim of this study was to evaluate a commercial sensor-a three-axis accelerometer-to predict animal behavior with a variety of conditions in tropical grazing systems. The sensor was positioned on the underjaw of young bulls to detect the animals' movements. A total of 22 animals were monitored in a grazing system, during both seasons (wet and dry), with different quality and quantity forage allowance.

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Knowledge of animal behavior can be indicative of the well-being, health, productivity, and reproduction of animals. The use of accelerometers to classify and predict animal behavior can be a tool for continuous animal monitoring. Therefore, the aim of this study was to provide strategies for predicting more and less frequent beef cattle grazing behaviors.

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Two experiments were conducted to evaluate the effect of replacing cottonseed meal with DDG on ruminal parameters, methane (CH) emissions (Experiment 1), and animal performance (Experiment 2) of young Nellore bulls grazing Marandu grass during the rainy season. Four supplementation strategies were used in both experiments: (1) Mineral supplementation (MS); (2) conventional multiple supplement (energy/protein) with cottonseed meal and citrus pulp (CMS); (3) CMS with 50% cottonseed meal replaced by DDG (50DDG); and (4) CMS with 100% cottonseed meal replaced by DDG (100DDG). The 50DDG condition resulted in greater intake of dry matter ( = 0.

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The effects of nitrogen (N) fertilization levels on protein and carbohydrate fractions in Marandu palisadegrass pasture [Urochloa brizantha (Hochst. ex A. Rich.

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Vitamin B and trace minerals are crucial molecular signals involved in many biological pathways; however, their bioavailability is compromised in high-producing ruminant animals. So far, studies have mainly focused on the effects of these micronutrients on animal performance, but their use in a rumen-protected form and their impact on liver metabolism in finishing beef cattle is poorly known. We used a shotgun proteomic approach combined with biological network analyses to assess the effects of a rumen-protected B-vitamin blend, as well as those of hydroxy trace minerals, on the hepatic proteome.

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The study aimed to evaluate the effect of replacing cottonseed meal by dried distiller's grains (DDG) in terms of efficiency in the productive aspects of beef cattle finishing in pasture versus feedlot. The experiment was conducted in a completely randomized design in a 2 × 3 factorial arrangement, with two production systems (pasture versus feedlot) and three supplements: CM, conventional supplement with cottonseed meal (CM) as a protein source; 50DDG: supplement with 50% replacement of CM by DDG; and 100DDG: 100% replacement. The effect of replacing CM by DDG on dry matter and nutrients intake and nutrients digestibility depends on the finishing system ( < 0.

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The effect of pasture management on CH emissions was investigated from goats in a tropical climate. Two experiments were conducted in a "Tanzania Guinea grass" (Panicum maximum Jacq.) pasture to assess enteric CH production in a completely randomized design.

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Cattle excreta and nitrogen (N) fertilizer deposited on tropical grasslands are important sources of ammonia (NH ) emission. We conducted three field trials (wet, intermediate, and dry conditions) to quantify NH emissions from urea fertilizer and simulated excretions of heifer urine and dung on warm-climate grasslands in Brazil. Heifer excreta were derived from pastures of palisadegrass [Urochloa brizantha (Hochst.

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This study aimed to evaluate different scenarios (year, supplementation level) about economic results of beef cattle production during rearing and finishing phase in Brazilian's tropical pastures. Four scenarios were evaluated in combination with fourteen supplements, and it was originated from some research developed inside Forage Crops and Grasslands section from São Paulo State University among years 2011 and 2014. The economic evaluation was analyzed by operating cost, total operational costs, gross revenue, operating profit, and financial net income.

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Two studies were carried out, the first with the objective to evaluate performance, beef quality and expression of genes involved in lipid metabolism in the muscle of bulls fed with or without low-fat dried distillers grains with solubles (DDGS, 21% DM) in the diet. In the second, eight rumen-fistulated bulls were assigned in a switch back design to evaluate the fatty acid profile of omasal fluid. We hypothesized that bulls fed DDGS may have an improved fatty acid profile and expression of genes involved in lipid metabolism may be altered, without affecting performance.

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The increase in haylage production leads to the search for additives that improve its fermentation and nutritional value. This study aimed to assess the effect of adding crude glycerine and microbial additives on losses, fermentation parameters and nutritional value of haylage. The treatments were composed of three doses of crude glycerine (0, 60 and 120 g/kg forage) and three types of inoculation (control (distilled water), SIL (Lactobacillus plantarum 2.

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Livestock production systems are an essential agribusiness activity in Brazil, but a critical challenge of Brazilian farmers is to maintain the equilibrium of the ecosystem, using herbage resources efficiently with a minimum impact on the environment. Nitrogen (N) fertilization and the inclusion of forage legumes into tropical grass pastures are management strategies which increase the productivity and nutritive value of pastures and may also affect methane (CH4) production by ruminants. The objective of this study was to examine the effects of either fertilizing palisade grass pastures with N or including the forage peanut (Arachis pintoi) into grass pastures on enteric CH4 emission, microbial protein production in the rumen via purine derivatives in the urine, and N balance.

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