[Evaluation of significance of Borrelia garinii spirochete recombinant protein DbpB for serodiagnostics of ixodes tick-borne borreliosis].

Aim: Obtaining recombinant protein DbpB of West Siberia Borrelia garinii 20047 isolate and evaluation of its antigen activity for the possible use in serodiagnostics of ixodes tick-borne borreliosis (ITB).

Materials And Methods: Coding region of dbpB gene of novosibirsk B. garinii 20047 isolate was amplified by PCR and cloned as part of expressing pETm vector in Escherichia coli BL21 (DE3) strain cells.

[Immunochemical analysis of recombinant protein FlaA of Western Siberian Borrelia garinii isolate].

Aim: Study of the ability of Western Siberian Borrelia garinii 20047 isolate recombinant protein FlaA to react with sera antibodies of ixodes tick borreliosis patients.

Materials And Methods: Recombinant antigen FlaA, sera of ixodes tick borreliosis patients, genetic engineering methods, solid phase EIA, and parametric and nonparametric statistical methods were used in the study.

Results: Recombinant form of mature flagellar protein FlaA of B.

[Enzyme immunoassay-based analysis of OspC recombinant proteins from Borrelia garinii and Borrelia afzelii isolated in West Siberia].

Aim: To study the ability of OspC recombinant proteins from Borrelia garinii and Borrelia afzelii isolated in West Siberia to interact with serum antibodies from patients with tick-borne borreliosis (TBB).

Materials And Methods: Recombinant antigens OspC B. garinii and OspC B.

[Characteristics of wheat powdery mildew growth along and across the longitudinal axis of a leaf under the action of exogenous zeatin].

Scanning electronic microscopy was used to investigate the regularities of growth direction of infectious structures and colonies of the agent of powdery mildew of wheat Erysiphe graminis f. sp. tritici.

[Influence of exogenous cytokinins on dynamics of development and differentiation of infection structure of the causal organism of wheat powdery mildew].

Using methods of light and electron scanning microscopy, development and infection structures differentiation of the causal organism of wheat powdery mildew, Erysiphe graminis DC. f. sp.

[Isolation of the recombinant proteins OspC and the fragment FlaA (F-FLAA) from the western-Siberian Borrelia garinii NT29 isolates and the study of their immunochemical properties].

The structural proteins OspC and FlaA of the Lyme disease (LD) agent are known to be the basic antigens, which induce the humoral immune response at the initial stage of the disease. The goal of this work was to obtain the recombinant OspC and a fragment of the FlaA protein (f-FlaA) from the Western Siberian Borrelia garinii NT29 isolates and to assess the possibility of their use for the LD diagnosis. Encoding regions of the OspC and f-FlaB genes were amplified using PCR inserted in the pREB expressive vectors and cloned in the E.

[Morphological variability of the wheat powdery mildew in the context of its parasitic adaptation to the lines of wheat Aegilops with different resistance].

We studied the interaction between the pathogen and wheat-Aegilops lines with different resistance as well as their parental forms in the course of powdery mildew infection using scanning electron microscopy. Line 51/99i proved to be similar to the parental form, Rodina variety, by the infection progress. The both genotypes featured pronounced adhesion of the primary infection structures to the surface of the plant epidermal cells in addition to formation of large developed colonies of the fungus, which indicates the partners compatibility.

[Characteristics of halo formation during pathogenesis as a response of cereal epidermal cells to penetration of powdery mildew pathogens].

A cytophysiological study was carried out of the functional status of a halo as a response of the host plant to contact with a powdery mildew pathogen. Interactions of the powdery mildew causative agents with barley, wheat, wheat-wheat-grass hybrids, wheat-aegilops lines, and aegilops with different genotypic resistance lead to the expression of haloes during pathogens, which are induced by infection pegs of the primary growth tubes appressoria, and hyphal lobes. Haloes are visualized using cytochemical reactions to proteins and scanning electron microscopy.