Iron-Catalyzed Reductive Ring-Rearrangement Reaction of Bridged Benzo[]oxocin-4-ones with Grignard Reagents to Afford Bridged Benzo[]oxocin-2-ols.

An iron-catalyzed reductive ring-rearrangement reaction of bridged benzo[]oxocin-4-ones with Grignard reagents to produce bridged benzo[]oxocin-2-ols is reported. Mechanistic studies indicate that an iron redox catalysis cycle involving oxidative addition to the C-O bond by low-valence iron and β-methoxyl elimination as key steps operates in this reaction.

Syntheses, crystal structures and intermolecular interactions of six novel pyrimidin-2-yl-substituted triaryltriazoles.

Six new pyrimidin-2-yl-substituted triaryltriazoles, namely, 4-(4-R-phenyl)-3-(pyridin-2-yl)-5-(pyrimidin-2-yl)-1,2,4-triazoles [L: R = methoxy (OCH); L: R = methyl (CH); L: R = nil (H); L: R = bromo (Br); L: R = chloro (Cl); L: R = fluoro (F)] have been successfully synthesized with yields in the range 68.3-81.7%.

OsAREB1, an ABRE-binding protein responding to ABA and glucose, has multiple functions in Arabidopsis.

Expression patterns of OsAREB1 revealed that expression of OsAREB1 gene can be induced by ABA, PEG and heat. Yeast one-hybrid assay demonstrated it can bind to ABA-responsive element (ABRE), which was found in most stress-induced genes. Transgenic Arabidopsis over-expressing OsAREB1 had different responses to ABA and glucose compared to wild-type plants, which suggest OsAREB1 might have a crucial role in these two signaling pathways.

[Cloning and bioinformatic analyzing of transcription factor AP2/ERF-B3 subfamily genes from Brassica napus L. Huyou 15].

AP2/ERF is a large family of transcription factors in plant. Genes in the AP2/ERF family encode transcriptional regulators with a variety of functions involved in the developmental and physiological processes in plants. Two AP2/ERF family transcriptional regulators (BnaERFB3-1 and BnaERFB3-2) were isolated from B.

[Cloning and bioinformatic analysis of the cDNA encoding a novel heat-shock factor OsHSF13 in Oryza sative L].

As a crucial transcription factor family,heat-shock factors were mainly analyzed and characterized in tomato and Arabidposis. In this study, we isolated a putative heat-shock factor OsHSF13 that interacted specifically with heat-shock element (HSE) from Oryza sativa L. by yeast one-hybrid method.

[Suppression of apple polyphenol oxidase by double-stranded RNA (RNAi)].

Antisense and sense gene fragments (710 base pairs) of apple polyphenol oxidase (APPO) gene were obtained by RT-PCR amplification, using the total RNAs isolated from ripen apple fruit as the template. These two fragments were ligated with a 1000 bp spacer, YYT (crtW+crtY fusion) gene, which is relative to carotenoid synthesization in subcocci. The full-length 2446 bp-target gene was then inserted into plant binary vector pYPX145 to generate the recombinant plasmid pYF7704, which carried the expression unit, of APPO dsRNA.

[Double-antisense ACC oxidase and ACC synthase fusion gene introduced into tomato by Agrobacterium-mediated transformation and analysis the ethylene production of transgenic plants].

The tomato fruit-specific promoter 2A11 was amplified from tomato genomic DNA using PCR techniques. Total RNA was isolated from ripen fruit of tomato, then ACC oxidase gene and ACC synthase gene were obtained using reverse-transcription polymerase chain reaction. The fusion encoding ACC oxidase and ACC synthase gene was obtained through ACC oxidase gene and ACC synthase gene ligation.