Publications by authors named "Reza Tafteh"
Single-particle tracking (SPT) is a powerful method for exploring single-molecule dynamics in living cells with nanoscale spatiotemporal resolution. Photostability and bright fluorescence make quantum dots (Qdots) a popular choice for SPT. However, their large size could potentially alter the mobility of the molecule of interest.
View Article and Find Full Text PDFArticle Synopsis
- - Single-molecule localization microscopy (SMLM) is crucial for studying biological structures, but its long acquisition time can lead to drift artifacts.
- - An innovative optical design using an electrically tunable lens (ETL) significantly reduces mechanical drift, achieving high precision in imaging (RMS ~0.7 nm lateral and ~2.7 nm axial).
- - The ETL system allows for better imaging of transferrin receptors in B-lymphocytes, revealing distinct cluster patterns, and is also budget-friendly and compatible with current microscopy setups.
A novel 3D imaging system based on single-molecule localization microscopy is presented to allow high-accuracy drift-free (<0.7 nm lateral; 2.5 nm axial) imaging many microns deep into a cell.
View Article and Find Full Text PDF