[Diagnosis of adult-onset Still's disease: contribution of biology].

We report one case of adult-onset Still's disease. The diagnosis of this disease currently requires application of Yamaguchi's criteria and then exclusion of infectious, haematological, autoimmune and neoplasic disorders. The place of biology is enhanced with the use of new markers like ferritin and glycosylated ferritin.

[Multicenter evaluation of thr Abbott glycosylated hemoglobin assay on IMx].

The Abbott IMx glycated hemoglobin assay was evaluated in a multicentre study. This method utilizes boronate affinity chromatography, and ion-capture technology. This assay determines both total glycohemoglobin (% GHb) and percentage of hemoglobin Alc (% HbAlc).

Comparison of six serum ferritin immunoassays and isoferritin spectrotypes in malignancies.

Serum ferritin was measured by six enzyme immunoassays in specimens from patients with digestive cancers (n = 30) and hematologic malignancies (n = 33). Most mean comparisons show significant differences in both groups of patients. In digestive cancers correlations between any two methods are very satisfactory (r > 0.

Serum ferritin and isoferritins are tools for diagnosis of active adult Still's disease.

Objective: Still's disease is an acute systemic inflammatory disorder. There are no pathognomonic symptoms or specific laboratory abnormalities. Serum ferritin concentration in rheumatoid arthritis together with some plasma glycoproteins such as alpha 2-glycoprotein and C-reactive protein are part of the response to inflammation.

[Multicenter study of sialic acid deficient transferrin determined by two chromatographic techniques].

Serum carbohydrate-deficient-transferrin (CDT) was measured by a micro anion-exchange chromatography/enzyme immunoassay. Results obtained on 245 sera analyzed in four laboratories were compared. Moreover, one laboratory used a commercial kit with ready-to-use microcolumns and a radioimmunoassay for measuring eluted CDT.

[Erythrocyte ferritin].

Red cell ferritin is a residue of erythroblast ferritin. It reflects the balance between the iron supply to the erythroid marrow and the need for haemoglobin synthesis. Erythrocyte ferritin can be measured in haemolysates after discarding plasma and leucocytes by different methods.

[Hemodialysis and ferritinemia].

Iron-deficiency is a common phenomenon in chronic renal diseases and haemodialysis patients, a treatment with iron or transfusions is always provided in an early preventive way; yet, an overload may appear. Serum ferritin, in spite of analytic variability, remains at the present time a good witness to appreciate patients' iron stores. Authors report the results obtained with four commercial reagents in healthy population and in haemodialysed children or adults.

Modification of the selected method for the determination of serum iron. Substitution of bathophenanthroline by ferene S.

The previously selected method (1977) for the measurement of iron in human serum has been modified by the working group on Iron and Iron-Transport Proteins (Sociéte Française de Biologie Clinique). Ferene S is used as the chromogen, it is more sensitive and cheaper than bathophenanthroline. The sample size has been reduced from 2 to 0.

[Reference values of erythrocyte ferritin in children and adults].

The erythrocyte ferritin content was measured in 183 healthy subjects in age from 4 to 68 years; 80 were male and 103 were female. In children between the ages of 4 and 12 years there is no significant difference in the mean value between boys and girls. In females the erythrocyte ferritin concentration is independent of age.

[The coefficient of saturation of iron in transferrin].

Theoretical iron fixation capacity of transferrin (FCT) can be calculated on its immunochemical titration: (FCT (mumol/l = transferrin (g/l) x 25). Today, its reckoning is more advisable to serum total iron binding capacity measurement. The authors studied the effects of this new proceeding upon usual values interval of transferrin saturation (i.

[Carcinoembryonic antigen. Evaluation in intestinal lavage fluids and in the tissue of colorectal tumors].

Carcinoembryonic antigen in pre-operative or pre-endoscopic intestinal lavage rinsing fluids was assayed in 21 healthy subjects, 15 presenting polyps and 12 presenting colorectal cancer. Comparison of the results with those of histological and immunohistological studies showed that this assay did not allow detection of intestinal cancer or evaluation of its developmental stage.

Determination of serum ferritin by a one-step immunoenzymoassay, and comparison of four liver-ferritin standards.

We describe a one step "sandwich"-type immunoenzymoassay for ferritin in human serum. The solid-phase consists of glutaraldehyde-treated polypropylene tubes coated with rabbit antibody to human ferritin. Liver ferritin is the standard.

[Carcinoembryonic antigen in intestinal lavage fluids and the tissue of colorectal tumors].

Determination of carcinoembryonic antigen (CEA) was carried out in 48 intestinal washing fluids (21 from healthy subjects, 15 from patients with polyps and 12 from patients with colorectal carcinoma). The histologic study of polyps and the histologic and immunohistochemical studies of tumors were realized. In the polyps's group, the mean of ACE is significantly higher than that of the volunteers's group, but 7 subjects have a CEA level below the mean and 8 subjects have a CEA level twice this mean.

[A new 1-step immunoenzyme sandwich technic. Application to the determination of total serum IgE].

A sandwich enzyme immunoassay by a one step procedure for total IgE in human serum is described. This method is a modification of a two steps sandwich procedure which uses glutaraldehyde-treated tubes and antibody conjugated with peroxidase. The procedure described is characterized by immune reactions performed in one step for one hour and by a substrate solution containing orthophenylene diamine.

[Measurement of peroxidase activity with orthophenylenediamine as chromogen in immunoenzyme methods].

The authors studied orthophenylenediamine in order to determine the optimal conditions for its use in "sandwich" type of enzymo-immunology. They studied the kinetics of the reaction and the stopping of the enzymatic reaction in the presence of peroxidase-labeled antibody. Studies of the dissolution of orthophenylenediamine and the stability of the solutions obtained show that a solution of orthophenylenediamine can be stored at ambiant temperature and in darkness for 8 hours.

"Sandwich" enzyme immunoassay for serum ferritin with polypropylene test tubes as the solid phase.

A "sandwich" enzyme immunoassay for ferritin in human serum is described, in which horseradish peroxidase-labeled antibody and a very sensitive chromogen, 2,2'-azino-di(3-ethyl-benzthiazolin-6-sulfonate), are used. The solid phase is polypropylene test tubes treated with glutaraldehyde; immunologic reactions are developed during constant rotation of the tubes. The assay requires 20 microL of serum per assay and takes less than 3 h.

[Study of present possibilities of interlaboratory standardisation of the prothrombin time (author's transl)].

The graphs or tables proposed by the manufacturer of reagents, the Thivolle straight lines suggested by Raby or, since 1977, Loeliger's procedure, are three possibilities offered to biologists to transform the prothrombin time into prothrombin concentrations expressed as a percentage. We have studied the possibility of interlaboratory standardisation in the case of patients treated with coumarin derivatives: firstly with the same thromboplastin under various conditions of use; secondly with five different thromboplastins under identical conditions (choosing arbitrarily one of the reagents as reference). Loeliger's procedure is the one which permits the best standardisation, but its practical application seems difficult.

[Hereditary bisalbuminemia. Study of a new familial case in France (author's transl)].

A case of bisalbuminemia of slow type was discovered in a 41 year old woman with obesity, diabetes mellitus and hypertension. This abnormality was discovered in six other members of the family and was thus hereditary. After a description of the laboratory tests used, the various forms of bisalbuminemia are described together with the circumstances of onset of the acquired forms, the biochemical and metabolic characteristics of the hereditary forms and their mode of transmission.