Angiopoietin-like protein 4: development, analytical characterization, and clinical testing of a new ELISA.

The aim of our work was to develop an assay for the determination of angiopoietin-like protein 4 (Angplt4) in human blood, and to investigate its levels in healthy volunteers and donors suffer from metabolic syndrome. We developed and evaluated the sandwich ELISA method for the quantitative determination of human Angplt4 in serum samples. We conducted also the pilot study on individuals with metabolic syndrome or familiar hypercholesterolemia and healthy probands and measured blood pressure, waist circumference, Angplt4 serum levels, serum cholesterol, triglycerides, HDL-cholesterol, LDL-cholesterol, insulin, glucose, A-FABP and calculate BMI and QUICKI insulin sensitivity index.

Determination of serum zinc-alpha-2-glycoprotein in patients with metabolic syndrome by a new ELISA.

Objectives: To develop an assay for the determination of ZAG in human serum, and to investigate its clinical relevance as a marker of metabolic syndrome.

Design And Methods: A new sandwich ELISA was introduced and clinically tested.

Results: ZAG serum level did not differentiate healthy subjects (27.